| 研究実績の概要 |
We have preliminary completed all whole genome comparative analyses among Sulawesi medaka species as follows: 1. Reconstructed the species trees for all medaka species/populations in Sulawesi Islands. 2. Computed absolute genetic distance analyses between the genomes of all medaka species pairs in Sulawesi. 3. Identified segregated sites between brooder and non-brooder species. 4. Completed synteny analyses between Sulawesi medaka species and outgroup species for each of the segregated sites found above. 5. Performed phylogenetic analyses and topological searches across all syntenic regions with segregated sites between brooders and non-brooder species. 6. Characterized the reproductive mode of all medaka species endemic to Sulawesi, and two outgroups. 7. Cross the information from my previous QTL analyses experiments on pelvic fin brooding with the results from whole-genome comparisons and segregated sites analyses. 8. Introgression analyses has been completed using genome wide SNPs and standard admixture statistical test.
The repeated evolution of pelvic fin brooding could be potentially explained by one of the following hypotheses: 1. Introgression, 2. Ancestral polymorphism, 3. Convergent evolution. Preliminary results reject the possibility for introgression from genus Adrianichthys to pelvic-fin brooder species in Oryzias, and the idea of ancestral polymorphism has been rejected as well. The major body of evidence support the view of a pure case of independent convergent evolution of pelvic-fin brooding in both clades.
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