| 研究課題/領域番号 |
19K16261
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| 研究機関 | 九州大学 |
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研究代表者 |
LEIWE MARCUS 九州大学, 医学研究院, 助教 (80722008)
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| 研究期間 (年度) |
2019-04-01 – 2022-03-31
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| キーワード | Olfactory Bulb / Development / Pruning / Odour |
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| 研究実績の概要 |
We began by assessing the olfactory responses generated in neonatal mice. We were able to reliably record M/T cell responses from mice ranging from P2 to the adult using 2-Photon Ca2+ imaging. We assessed the difference between P2 and P6 to see if the sensitivity of glomeruli were different. P2 mice were slightly more broadly tuned than P6 mice. However, the responses were much stronger at P6 perhaps meaning that, we were able to detect more smaller events rather than actually observing a change in odour tuning.
Progress was made in being able to alter the phase code of neonatal mice. With a global KO of DA cells the consistency of the phase code was decreased. We have constructed a Cre-specific Tetanus AAV vector to see if we can reproduce these mice in healthier mice.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
4: 遅れている
理由
Our 2-Photon has broken, meaning we are unable to continue with our calcium imaging experiments. However, funding seems to have been procured to purchase a new pulsing laser meaning experiments can continue soon.
Our AAV vector has been constructed and is ready for use once the laser has been repaired
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| 今後の研究の推進方策 |
1) Trial the lower concentration of odours at P2 to P6
2) Record the changes in phase coding after the injection of pAAV-Flex-TeNT-p2A-mRuby3
3) Come up with an alternative strategy if point 1) fails.
4) Try to find a way to prevent the pruning of mitral cells and see if there is an effect
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| 次年度使用額が生じた理由 |
Due to the pandemic and our 2-Photon failing it was impossible to do experiments for a long while. I'm only just begining to recover the time lost.
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