| 研究実績の概要 |
The research achievements are as follows- (1) We established a high-quality genome assembly of camptothecin producing plant Ophiorrhiza pumila, one of the best plant genomes assembled ever. (2) We identified critical enzymes involved in the biosynthesis of camptothecin. (3) We established a pan-genome for monoterpene indole alkaloids biosynthesis using whole-genome assemblies of O. pumila, Catharanthus roseus, Camtotheca accuminata, and Gelsemium sempervirens. (4) We established the whole-genome assembly of Ophiorrhiza japonica, a close species of Ophiorrhiza pumila, which does not produce camptothecin. (5) We also established whole genome assembly for Nothapodytes foetida, which has a genome size of 9.79Gb. (6) In this study, we established metabolome resources to expand the study on camptothecin biosynthesis using complete carbon and nitrogen labeling approaches. (7) In this study, we established the emergence of STR (strictosidine synthase enzyme) as the key evolutionary event for the evolution of MIAs and camptothecin biosynthesis. Our results proposed a way to identify putative candidate genes associated with camptothecin biosynthesis. (8) We established STR knockout, an enzyme that catalyzes the biosynthesis of strictosidine. The line established in this study will be used to identify candidate genes associated with camptothecin biosynthesis, which will be used for further functional characterization. (9) We performed transcriptome analysis using Ophiorrhiza pumila hairy roots for four phytohormones across 8-time points. The coexpression analysis is ongoing.
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