| 研究実績の概要 |
We speculated declined muscle self-renewal with aging is mediated partially by the O-GlcNAcylation on Notch1.
To understand if the O-GlcNAcylation status of Notch1 is altered with aging, I established the inducible FLAG-Notch1 transgenic mice. Afterwards, O-GlcNAcylation on Notch1 protein extracted from aged and younger mice will be applied in glycosylation analysis.
Albeit Eogt didn’t be detected in satellite cells of muscle, I found Eogt distributes only in “certain” myofibers. In further experiments, I found Eogt highly overlaps myofiber type2A.
The mouse hanging assay also shows Eogt knockdown reduces muscle contractibility.
It suggests that Eogt is involved in muscle contractibility by mediating myofiber types. This study facilitates to ameliorate mobility of aged people.
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