| 研究課題/領域番号 |
19K16520
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| 研究機関 | 広島大学 |
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研究代表者 |
阿久津 シルビア夏子 (AkutsuSilviaNatsuko) 広島大学, 原爆放射線医科学研究所, 助教 (10822299)
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| 研究期間 (年度) |
2019-04-01 – 2022-03-31
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| キーワード | iPS cells / Chromosome / Aneuploidy / Reprogramming / Down syndrome |
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| 研究実績の概要 |
Down's syndrome is the most prevalent chromosomal aneuploidy caused by trisomy 21, but its curative cure has not yet been established. Recently, the possibility of a "chromosomal aneuploidy therapy" in which trisomy 21 is rescued was demonstrated by reprogramming iPS cells. Here, I proposed to reproduce this approach and increase the aneuploidy rescue efficiency that was shown as extremely low, being a major obstacle for future basic research and clinical application of aneuploidy rescue. Currently, I am establishing a highly efficient chromosomal aneuploidy rescue experimental protocol for trisomy disorders.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The highly efficient chromosomal aneuploidy rescue experimental protocol is being established for trisomy 21 and another trisomy disorders. Successfully, primary fibroblasts from trisomy disorders showed karyotype rescue after reprogramming to iPS cells. The Fluorescence In situ Hybridization (FISH) and karyotype analysis were performed to confirm the results. The reproducibility results from previous studies have been successfully obtained. The results of this research will be an epoch-making basic technology for the root cure of aneuploidy diseases.
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| 今後の研究の推進方策 |
The next step in the research is to test protocols for rescuing aneuploidies in different cell lines of the same aneuploidy diseases. When the protocol is well established, it will be possible to begin the investigation of the trisomy rescue mechanism during cellular reprogramming.
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| 次年度使用額が生じた理由 |
The next fiscal year, I plan to construct a new detection system targeting different fluorescent dye-expressing genes on chromosome 21 of Down syndrome iPSCs by genome editing to evaluate the rescue of trisomy in the reprogramming process. The results will be presented in the Annual Meeting Society of Genetics.
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