研究課題/領域番号 |
19K16531
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研究機関 | 北海道大学 |
研究代表者 |
陳 震 北海道大学, 保健科学研究院, 助教 (60802634)
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研究期間 (年度) |
2019-04-01 – 2021-03-31
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キーワード | oxdation stress / oxidized lipids / lipid hydroperoxides / lipidomics / plasmalogen / antioxidant / LC/MS |
研究実績の概要 |
We have developed the evaluation system of both biology and chemistry. By using the human hepatocyte as a pilot study, we tested oxidation stress model and its potential protection trial. We chose the heavy metal lead (Pb) stimulation as the oxidation model, and used the bioactive phospholipid plasmalogen as the antioxidant. The present work revealed that Pb-mediated oxidative stress reduced the HepG2 cell viability in association with the generation of ROS, the hydroperoxides as the oxidized phospholipids, and the lysophospholipids as the degradation products. These adverse effects of Pb were largely eliminated by plasmalogen species with different headgroups and sn-2 fatty acyls via both a sacrificial trap for ROS and a mechanistic activation of Nrf-2-dependent antioxidant enzymes.
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現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The evaluation system, including the cell culture, the ROS and antioxidant enzyme assay, the lipidomic tests of cells, have been established.
The nerve cells (PC12) have been purchased and cultured.
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今後の研究の推進方策 |
We will apply the current workflow onto the PC12 cells. Because nerve cells are different from liver cells, in the following experiment we will notice any new findings and specific characteristics.
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