| 研究課題/領域番号 |
19K18802
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| 研究機関 | 信州大学 |
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研究代表者 |
デイ ティモシー 信州大学, 医学部, 研究員 (00838667)
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| 研究期間 (年度) |
2019-04-01 – 2021-03-31
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| キーワード | KCNQ4 / model mice / hearing loss |
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| 研究実績の概要 |
Disabling hearing loss is one of the most frequent disorders that affects 5% of the world’s population, and half of congenital hearing loss is caused genetically. KCNQ4 encodes for a potassium channel which maintains potassium concentrations surrounding the hair cells, the sensory cells in the inner ear. Dysregulation in potassium concentrations gradually damages the hair cells, resulting in hearing loss.
In this year, we quantitatively measure the auditory phenotype of newly developed KCNQ4 mutation knock in model mice, objectively using the auditory brain stem response (ABR) and otoacoustic radiation (OAE). Morphology is verified by scanning electron microscope and transmission electron microscope in addition to fluorescent immunostaining also starting.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We can quantitatively measure the auditory phenotype of newly developed KCNQ4 mutation knock in model mice, objectively using the auditory brain stem response (ABR) and otoacoustic radiation (OAE) .
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| 今後の研究の推進方策 |
We will also perform the auditory measurement of newly developed KCNQ4 mutation knock in model mice, objectively using the auditory brain stem response (ABR) and otoacoustic radiation (OAE). We will also perform in vitro experiment for phenotypic analysis of KCNQ4 mutation.
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| 次年度使用額が生じた理由 |
当初計画していたのと比較しマウスの繁殖が悪く、形態の観察が次年度以降になり観察のための試薬代金が繰越しとなった。現在、順調に繁殖するようになったため次年度以降に研究を実施する
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