| 研究実績の概要 |
TECs and NECs normal endothelial cells were successfully isolated. The metabolomes in each cell type were analyzed by CE-MS metabolomics. Both unlabeled and carbon-13 labeled glutamine were used together with unlabelled glucose as major substrates. The metabolites within the cells (intracellular) and metabolites released into the culture media (extracellular) were analyzed. NECs converted glutamine primarily to GABA, whereas tumor endothelial cells produced more glutamate, asparagine, and aspartate from glutamine. In this year, I have obtained the results as described below.
1.Normal endothelial cells and tumor endothelial cells have different metabolic endpoints for glutamine - NECs produce lots of GABA, whereas TECs produce more metabolic intermediates such as glutamate, asparagine. 2.Glycolysis (extracellular acidification) is higher in TECs produce than in NECs, while oxygen consumption and spare respiratory capacity are decreased in the TECs compared to the NECs.
3.Inhibition of the glutamate-cysteine antiporter Slc7a11 with the drug sulfasalazine leads to a significant decrease in TEC proliferation compared to NECs.
4.TECs are more dependent on a functional antioxidant system to continue proliferation; therefore, in the presence of cystine or cysteine, the adverse effects of sulfasalazine were abrogated.
5.Sulfasalazine treatment decreased tumor weight and tumor angiogenesis in tumor-bearing mice. 6.Changes in the extracellular matrix and tumor microenvironment were not significantly different between the treated tumor-bearing mice and their controls.
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