研究実績の概要 |
By using the new generation laminin (NGL), which was conjugated heparan sulfate chains (HS) to Laminin-E8 fragments, we established a Xeno-free system for differentiating myocytes and muscle stem cells from hiPSCs. In the step-wised protocol, NGL time-dependently increases the populations of primitive streak, paraxial mesoderm, dermomyotome, muscle progenitors, myocytes, and then muscle stem cells. The mechanism is that HS bind to FGF2 in the culture medium, form a stable high affinity HS-FGF2-FGFR complexes on cell surface, then strongly stimulate FGFR signaling pathway at the starting point of cell differentiation. These effects depend on the unique structure of NGL, that could not be replaced by treating with high dose of FGF2 or coating with the mixture of laminin-E8 fragments and HS.
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