| 研究実績の概要 |
Ozone is a universal air pollutant with extremely high oxidative activity. The livings on the earth are hard to avoid the deleterious effect of ozone, because many organs, such as the respiratory airway and the skin, expose to the air directly. This study aims to set up a novel lipidomic methodology to detect the oxidized lipids during exposure to ambient level of ozone by high resolution LC/MS.
The main achievements of the first year are summaries as below:
(1) We studied the model lipid membrane system which is composed by the main components of the cell membrane, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and cholesterol. We successfully identified and quantified several kinds of ozonation products. We also found mixing cholesterol into the membrane can strongly influence the amount of certain ozonation products.
(2) We successfully set up a culture system with lung cell and ozone which is used to mimic the lung under oxidation of low-level ozone. We also identified some aldehyde from certain unsaturated lipids of the cultured cell as oxidation products after the ozone exposure.
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| 今後の研究の推進方策 |
In the next year, the plans are as follow:
1. The lipidomic methodology by LC/MS will be further optimized and utilized to quantifying the oxidized lipids.
2. The influencing factor of the oxidation reaction between lung cell and ozone will be further studied including the ozone concentration, the exposure time and the humility of the air.
3. The mechanism of this oxidation process will be further investigated by combining with other biochemical methods.
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