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2019 年度 実績報告書

精密な脂質分析を用いるヒト肺と皮膚細胞に対する環境濃度オゾンの影響に関する研究

研究課題

研究課題/領域番号 19K21122
配分区分基金
研究機関北海道大学

研究代表者

喬 琳  北海道大学, 保健科学研究院, 助教 (30821524)

研究期間 (年度) 2019-04-01 – 2020-03-31
キーワードlow-concentration ozone / ozonation / lipid oxidation / A549 cell / lung surfactant / lung damage / orbitrap LC-MS/MS / lipidomic methodology
研究実績の概要

Ozone has a very high oxidative activity with a concentration of a few tens of ppb in the ambient air. During breathing, the lipids and the cells in one’s respiratory system are hard to avoid the oxidative effect of ozone. Here, by exposing the lipid of lung surfactant and the human alveolar epithelial cells to the ambient ozone of a low concentration (20-100 ppb), we systematically studied the lipid oxidation through high resolution LC-MS/MS.
The lipid monolayers composed by DPPC, POPC and POPG were used as the model system of the lung surfactant. Their stability and ozonized products were successfully identified and quantified. By mixing with cholesterol, we observed the physicochemical properties of the lipid membrane strongly influenced the yield of certain ozonation products. Furthermore, we found a phenolic compound 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA) strongly inhabited the oxidation process.
In addition, we established a novel lipidomics method and successfully applied it to study the ozonation of the adenocarcinomic human alveolar basal epithelial cell (A549). We found the aldehyde-type ozonized lipids are the major products. More importantly, the secondary ozonides (SOZ) with the head group of phosphocholine were also identified as the products and its amount is related to the relative humility of the air.
Our study gave a molecular-level view of the effect of the ambient ozone on the lipids in the lung. The novel lipidomics method has great potential for searching the biomarker of the lung damage in the low-concentration air pollutant.

  • 研究成果

    (4件)

すべて 2020 2019

すべて 雑誌論文 (1件) (うち査読あり 1件、 オープンアクセス 1件) 学会発表 (3件)

  • [雑誌論文] Absorption, Fluorescence, and Two-Photon Excitation Ability of 5-Phenylisolidolo[2,1-a]quinolines2020

    • 著者名/発表者名
      Avena Ramon Francisco、Qiao Lin、Fujii Yuki、Otomo Kohei、Ishii Hirokazu、Suzuki Takeyuki、Tsujino Hirofumi、Uno Tadayuki、Tsutsumi Yasuo、Kawashima Yusuke、Takagi Tatsuya、Murai Kenichi、Nemoto Tomomi、Arisawa Mitsuhiro
    • 雑誌名

      ACS Omega

      巻: 5 ページ: 2473~2479

    • DOI

      10.1021/acsomega.9b04070

    • 査読あり / オープンアクセス
  • [学会発表] The Oxidation of Lipid Monolayers of Lung Surfactant in Low-level Ozone2019

    • 著者名/発表者名
      Lin Qiao, Chiba Hitoshi, Shen Ye, Shu-Ping Hui
    • 学会等名
      第61回 日本脂質生化学会
  • [学会発表] 質量分析を用いる肺サーファクタントリン脂質膜に対する環境濃度オゾンの影響に関する研究2019

    • 著者名/発表者名
      喬 琳, 陳 震, 高田 俊智, 千葉 仁志 ,  叶 深, 惠 淑萍
    • 学会等名
      第44回日本医用マススペクトル学会
  • [学会発表] オゾンによる肺サーファクタントリン脂質膜の構造と安定性への影響2019

    • 著者名/発表者名
      喬 琳, 陳 震, 高田 俊智,  千葉 仁志,  叶 深,  惠 淑萍
    • 学会等名
      第29回日本臨床化学会北海道支部例会

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公開日: 2021-01-27  

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