| 研究課題/領域番号 |
19K23728
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| 研究機関 | 広島大学 |
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研究代表者 |
IJAZ FARYAL 広島大学, 医系科学研究科(医), 助教 (80845595)
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| 研究期間 (年度) |
2019-08-30 – 2022-03-31
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| キーワード | Primary Cilium / Extracellular Vesicles |
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| 研究実績の概要 |
The aim of this study is to find the physiological role of primary cilium derived extracellular vesicles in acceptor cells. Proteomics analysis of the target cells after exposure to pcEVs was performed. The results showed the increase in proteins related to cell proliferation and metabolism in the target cells exposed to pcEVs which were in line with our previous findings of increased rate of migration in target cells after exposure to pcEVs. Furthermore, in order to know whether the above mentioned effects were truly from pcEVs, we optimized the method to purify pcEVs from the mixture of extracellular vesicles using exosome depletion and purification of pcEVs.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
the research project is going rather smoothly as we have already devised the method to purify pcEVs which is important for confirming the physiological effects of pcEVs in target cells.
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| 今後の研究の推進方策 |
The next plan is to do the proteomics and RNA analysis of the purified pcEVs using our recently optimized purification methods and confirmation of the results through biochemical and biological assays.
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| 次年度使用額が生じた理由 |
Due to the pandemic situation some aspects of the research project could not be done. The remaining amount will be used for final omics analysis and the confirmation of the omics results and for paper publication costs.
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