Generation of universal donor cell souse for adoptive T cell therapy using iPSC technology

2020 年度 実績報告書

• 研究課題/領域番号: 19K23863
• 研究機関: 京都大学
• 研究代表者: 王 博 京都大学, iPS細胞研究所, 研究員 (80842765)
• 研究期間 (年度): 2019-08-30 – 2021-03-31
• キーワード: immune rejection / iPSC-derived T cells / gene editing

研究実績の概要

We generated hypoimmunogenic cancer-antigen-specific T cells derived from induced pluripotent stem cells (iPSCs) lacking β2-microglobulin, the class-II major histocompatibility complex (MHC) transactivator and the natural killer (NK) cell-ligand poliovirus receptor CD155, and expressing single-chain MHC class-I antigen E. In mouse models of CD20-expressing B-Lymphoblastoid cells, differentiated T cells expressing a CD20 chimeric antigen receptor largely escaped recognition by NKG2A+ and DNAM-1+ NK cells and by CD8 and CD4 T cells in the allogeneic recipients while maintaining antitumour potency.

研究成果

• [雑誌論文] Generation of hypoimmunogenic T cells from genetically engineered allogeneic human induced pluripotent stem cells (2021)
  • 著者名/発表者名: Bo Wang, Shoichi Iriguchi, Masazumi Waseda, Norihiro Ueda, Tatsuki Ueda, Huaigeng Xu, Atsutaka Minagawa, Akihiro Ishikawa, Hisashi Yano, Tomoko Ishi, Ryoji Ito, Motohito Goto, Riichi Takahashi, Yasushi Uemura, Akitsu Hotta and Shin Kaneko
  • 雑誌名: Nature Biomedical Engineering 巻: in press ページ: in press
  • DOI: 10.1038/s41551-021-00730-z
  • 査読あり / オープンアクセス / 国際共著
• [学会発表] Universal T cell generation for off-the-shelf cancer immunotherapy from gene edited allogeneic iPSCs (2020)
  • 著者名/発表者名: Bo Wang, Shoichi Iriguchi, Masazumi Waseda, Norihiro Ueda, Tatsuki Ueda, Huaigeng Xu, Atsutaka Minagawa, Akihiro Ishikawa, Yasushi Uemura, Akitsu Hotta and Shin Kaneko
  • 学会等名: 第19回日本再生医療学会総会
• [備考] 京都大学iPS細胞研究所
  • URL: https://www.cira.kyoto-u.ac.jp/
• [備考] 金子研究室
  • URL: http://www.cira.kyoto-u.ac.jp/kaneko/index.html