| 研究課題/領域番号 |
19K24152
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| 研究機関 | 九州歯科大学 |
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研究代表者 |
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| 研究期間 (年度) |
2019-08-30 – 2021-03-31
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| キーワード | Sarcopenia / Transcription / Epigenetics / Muscle / Zfp423 |
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| 研究実績の概要 |
We have made significant progress in our understanding of the mechanisms by which Zfp423 regulates myoblast cell function. Using ChIP-Seq, we have constructed a detailed chromatin localization and occupancy map of Zfp423 in myoblast cells. This data has allowed us to identify several putative targets, define Zfp423 DNA-binding motifs, and candidate co-occupancy factors.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Currently, we are in the process of evaluating the effects of Zfp423 binding at putative targets in muscle cells. In addition, generation and characterization of Zfp423 conditional knockout mice is ongoing.
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| 今後の研究の推進方策 |
We will conditionally delete Zfp423 in in satellite cells using Pax7-Cre and perform a complete analysis of muscle and satellite cell functions in vivo and ex vivo at 3 weeks, 7 months and 18 months of age. Muscle mass, size and morphology will be analysed by histomorphometry and DEXA densitometry. Immunohistological staining will be used to quantify SC pool size and proliferative capacity. Ex vivo assessment of primary SCs and myofiber culture assays will be used to identify cell-autonomous changes in candidate signaling pathways and target gene expression.
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| 次年度使用額が生じた理由 |
We were able to reduce expenditure and costs of consumable materials by advancing our experiments in a more efficient manner.
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