腎臓膜輸送体を制御する新規細胞内刺激伝達系の解明

2012 年度 研究成果報告書

• 研究課題/領域番号: 20249047
• 研究種目: 基盤研究(A)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 腎臓内科学
• 研究機関: 東京医科歯科大学
• 研究代表者: 内田 信一 東京医科歯科大学, 大学院・医歯学総合研究科, 准教授 (50262184)
• 研究期間 (年度): 2008 – 2012
• キーワード: 水・電解質代謝学 / 塩分感受性高血圧 / 遺伝子改変マウス

研究概要

我々は、遺伝性高血圧症である偽性低アルドステロン症II(PHAII)の原因遺伝子であるWNKキナーゼのシグナル伝達系を、Wnk4D561A/+PHAII病態モデルを用いて明らかにした(CellMetab2007)。本研究は、PHAII以外にこの新規WNKキナーゼシグナルカスケードが生体内でもつ病態生理学的意義を明らかにすることを目的として行われた。その結果、WNKキナーゼを制御する液性因子や下流で制御される新たな輸送体を同定し、生体内におけるWNKキナーゼの血圧調整における重要な役割とその制御機構を明らかにした。

研究成果

• [雑誌論文] ImpairedKLHL3-Mediated Ubiquitination of WNK4 Causes Human Hypertension (2013)
  • 著者名/発表者名: Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, NomuraN, Mori Y, Matsuo H, Murata T, Nomura S, Asano T, Kawaguchi H, Nonoyama S, Rai T, Sasaki S, Uchida S
  • 雑誌名: Cell Rep 巻: 3(3) ページ: 858-68
  • 査読あり
• [雑誌論文] Involvement of aquaporin-7 inthe cutaneous primary immune response through modulation of antigen uptake and migration in dendritic cells (2012)
  • 著者名/発表者名: Hara-Chikuma M, Sugiyama Y, Kabashima K, Sohara E, Uchida S, Sasaki S, Inoue S, Miyachi Y
  • 雑誌名: FASEB J 巻: 26 ページ: 211-8
  • 査読あり
• [雑誌論文] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination (2012)
  • 著者名/発表者名: Hossain Khan MZ, Sohara E, Ohta A, ChigaM, Inoue Y, Isobe K, Wakabayashi M, Oi K, Rai T, Sasaki S, Uchida S
  • 雑誌名: Biochem. Biophys. Res. Commun 巻: 425 ページ: 456-61
  • 査読あり
• [雑誌論文] A new role for aquaporin 7 in insulin secretion (2012)
  • 著者名/発表者名: Louchami K, Best L, Brown P, Virreira M, Hupkens E, Perret J, Devuyst O, Uchida S, Delporte C, Malaisse WJ, Beauwens R, SenerA
  • 雑誌名: Cell Physiol. Biochem 巻: 29 ページ: 65-74
  • 査読あり
• [雑誌論文] ASK3 responds to osmotic stress and regulates blood pressure by suppressing WNK1-SPAK/OSR1 signaling in the kidney (2012)
  • 著者名/発表者名: Naguro I, Umeda T, Kobayashi Y, MaruyamaJ, Hattori K, Shimizu Y, Kataoka K,Kim-Mitsuyama S, Uchida S, Vandewalle A, Noguchi T, Nishitoh H, Matsuzawa A, Takeda K, Ichijo H
  • 雑誌名: Nat. Commun 巻: 3 ページ: 1285
  • 査読あり
• [雑誌論文] Phosphatidylinositol 3-kinase/Akt signaling pathway activates theWNK-OSR1/SPAK-NCC phosphorylation cascade in hyperinsulinemic db/db mice (2012)
  • 著者名/発表者名: Nishida H, Sohara E, Nomura N, Chiga M, Alessi DR, Rai T, Sasaki S, Uchida S
  • 雑誌名: Hypertension 巻: 60 ページ: 981-90
  • 査読あり
• [雑誌論文] Accumulation of autofluorescent storage material in brain is accelerated byischemia in chloride channel 3gene-deficient mice (2012)
  • 著者名/発表者名: Ohtaki H, Ohara K, Song D, Miyamoto K, Tsumuraya T, Yofu S, Dohi K, Tanabe S, Sasaki S, Uchida S, Matsunaga M, Shioda S
  • 雑誌名: J. Neurosci. Res 巻: 90 ページ: 2163-72
  • 査読あり
• [雑誌論文] A minor role of WNK3 in regulating phosphorylation ofrenal NKCC2 and NCC co-transporters in vivo (2012)
  • 著者名/発表者名: Oi K, Sohara E, Rai T, Misawa M, Chiga M, Alessi DR, Sasaki S, Uchida S
  • 雑誌名: Biol.Open 巻: 1 ページ: 120-7
  • 査読あり
• [雑誌論文] Effect of heterozygousdeletion of WNK1 on the WNK-OSR1/SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels (2012)
  • 著者名/発表者名: Susa K, Kita S, Iwamoto T, Yang SS, LinSH, Ohta A, Sohara E, Rai T, Sasaki S, Alessi DR, Uchida S
  • 雑誌名: Clin. Exp. Nephrol 巻: 16 ページ: 530-8
  • 査読あり
• [雑誌論文] WNK-OSR1/SPAK-NCC signal cascade has circadian rhythmdependent on aldosterone (2012)
  • 著者名/発表者名: Susa K, Sohara E, Isobe K, Chiga M, Rai T, Sasaki S, Uchida S
  • 雑誌名: Biochem. Biophys. Res. Commun 巻: 427 ページ: 743-7
  • 査読あり
• [雑誌論文] Does a β2-adrenergicreceptor-WNK4-Na-Cl co-transporter signal cascade exist in the in vivo kidney? (2012)
  • 著者名/発表者名: Uchida S, Chiga M, Sohara E, Rai T, Sasaki S
  • 雑誌名: Nat.Med 巻: 18 ページ: 1324-5
  • 査読あり
• [雑誌論文] Phenotypes of pseudohypoaldosteronism type II caused by the WNK4 D561A missense mutation are dependent on theWNK-OSR1/SPAK kinase cascade (2011)
  • 著者名/発表者名: Chiga M, Rafiqi FH, Alessi DR, Sohara E, Ohta A, Rai T, Sasaki S, Uchida S
  • 雑誌名: J. Cell Sci 巻: 124 ページ: 1391-5
  • 査読あり
• [雑誌論文] Impaired phosphorylation of Na(+)-K(+)-2Cl(-) cotransporter by oxidative stress-responsive kinase-1deficiency manifests hypotension and Bartter-like syndrome (2011)
  • 著者名/発表者名: Lin SH, Yu IS, Jiang ST, Lin SW, Chu P, Chen A, Sytwu HK, Sohara E, Uchida S, Sasaki S, Yang SS
  • 雑誌名: Proc. Natl. Acad. Sci. U S A 巻: 108 ページ: 17538-43
  • 査読あり
• [雑誌論文] Regulation of WNK1 kinase by extracellular potassium (2011)
  • 著者名/発表者名: Naito S, Ohta A, Sohara E, Ohta E, Rai T, Sasaki S, Uchida S
  • 雑誌名: Clin. Exp. Nephrol 巻: 15 ページ: 195-202
  • 査読あり
• [雑誌論文] Generation and analyses of R8L barttin knockin mouse (2011)
  • 著者名/発表者名: Nomura N, Tajima M, Sugawara N, Morimoto T, Kondo Y, Ohno M, Uchida K, Mutig K, Bachmann S, Soleimani M, Ohta E, Ohta A, Sohara E, Okado T, Rai T, Jentsch TJ, Sasaki S, UchidaS
  • 雑誌名: Am. J. Physiol. Renal. Physiol 巻: 301 ページ: F297-307
  • 査読あり
• [雑誌論文] Dexamethasone increases the phosphorylation of nephrin in cultured podocytes (2011)
  • 著者名/発表者名: Ohashi T, Uchida K, Uchida S, Sasaki S, Nitta K
  • 雑誌名: Clin. Exp. Nephrol 巻: 15 ページ: 688-93
  • 査読あり
• [雑誌論文] Immunolocalization of WNK4 in mouse kidney (2011)
  • 著者名/発表者名: Ohno M, Uchida K, Ohashi T, Nitta K, Ohta A, Chiga M, Sasaki S, UchidaS
  • 雑誌名: Histochem. Cell Biol 巻: 136 ページ: 25-35
  • 査読あり
• [雑誌論文] Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney (2011)
  • 著者名/発表者名: Sohara E, Rai T, Yang SS, Ohta A, NaitoS, Chiga M, Nomura N, Lin SH, Vandewalle A, Ohta E, Sasaki S, Uchida S
  • 雑誌名: PLoS One 巻: 6 ページ: e24277
  • 査読あり
• [雑誌論文] Phosphorylation of aquaporin-2 regulates its water permeability (2010)
  • 著者名/発表者名: Eto K, Noda Y, Horikawa S, Uchida S, Sasaki S
  • 雑誌名: J. Biol. Chem 巻: 285 ページ: 40777-84
  • 査読あり
• [雑誌論文] Short-term stimulation of the thiazide-sensitive Na+-Cl- cotransporter by vasopressin involves phosphorylation and membrane translocation (2010)
  • 著者名/発表者名: Mutig K, Saritas T, Uchida S, Kahl T,Borowski T, Paliege A, Bohlick A, Bleich M, Shan Q, Bachmann S
  • 雑誌名: Am. J. Physiol. Renal Physiol 巻: 298 ページ: 502-9
  • 査読あり
• [雑誌論文] Calcium and calcimimetics regulate paracellular Na+ transport in the thin ascending limb of Henle's loop in mouse kidney (2010)
  • 著者名/発表者名: Sugawara N, Morimoto T, Farajov EI, Kumagai N, Aslanova UF, Rai T, Uchida S, Sasaki S, Tsuchiya S, Kondo Y
  • 雑誌名: Pflugers Arch 巻: 460 ページ: 197-205
  • 査読あり
• [雑誌論文] Effect of angiotensin II on the WNK-OSR1/SPAK-NCC phosphorylation cascade in cultured mpkDCT cells and in vivo mouse kidney (2010)
  • 著者名/発表者名: Talati G, Ohta A, Rai T, Sohara E, Naito S, Vandewalle A, Sasaki S, Uchida S
  • 雑誌名: Biochem. Biophys. Res. Commun 巻: 393 ページ: 844-8
  • 査読あり
• [雑誌論文] Generation and analysis of the thiazide-sensitive Na+ -Cl- cotransporter (Ncc/Slc12a3) Ser707X knockin mouse as a model of Gitelman syndrome (2010)
  • 著者名/発表者名: Yang SS, Lo YF, Yu IS, Lin SW, Chang TH, Hsu YJ, Chao TK, Sytwu HK, Uchida S, Sasaki S, Lin SH
  • 雑誌名: Hum. Mutat 巻: 31 ページ: 1304-15
  • 査読あり
• [雑誌論文] SPAK-knockout mice manifest Gitelman syndrome and impaired vasoconstriction (2010)
  • 著者名/発表者名: Yang SS, Lo YF, Wu CC, Lin SW, Yeh CJ, Chu P, Sytwu HK, Uchida S, Sasaki S, Lin SH
  • 雑誌名: J. Am. Soc. Nephrol 巻: 21 ページ: 1868-77
  • 査読あり
• [雑誌論文] Mechanisms for hypercalciuria in pseudohypoaldosteronism type II-causing WNK4 knock-in mice (2010)
  • 著者名/発表者名: Yang SS, Hsu YJ, Chiga M, Rai T, Sasaki S, Uchida S, Lin SH
  • 雑誌名: Endocrinology 巻: 151 ページ: 1829-36
  • 査読あり
• [雑誌論文] Pathophysiological roles of WNK kinases in the kidney (2010)
  • 著者名/発表者名: Uchida S
  • 雑誌名: Pflugers Arch 巻: 460 ページ: 695-702
  • 査読あり
• [雑誌論文] Aquaporin-2 regulates cell volume recovery via tropomyosin (2009)
  • 著者名/発表者名: Li YH, Eto K, Horikawa S, Uchida S, Sasaki S, Li XJ, Noda Y
  • 雑誌名: Int. J. Biochem. Cell Biol 巻: 41 ページ: 2466-2476
  • 査読あり
• [雑誌論文] Targeted disruption of the Wnk4 genedecreases phosphorylation of Na-Cl cotransporter, increases Na excretion and lowers blood pressure (2009)
  • 著者名/発表者名: Ohta A, Rai T, Yui N, Chiga M, Yang SS, Lin SH, Sohara E, Sasaki S, Uchida S
  • 雑誌名: Hum. Mol. Genet 巻: 18 ページ: 3978-86
  • 査読あり
• [雑誌論文] Pancreas-specific aquaporin 12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis (2009)
  • 著者名/発表者名: Ohta E, Itoh T, Nemoto T, Kumagai J, Ko SB, Ishibashi K, Ohno M, Uchida K, Ohta A, Sohara E, Uchida S, Sasaki S, Rai T
  • 雑誌名: Am. J. Physiol. Cell Physiol 巻: 297 ページ: 1368-78
  • 査読あり
• [雑誌論文] FAPP2 is required for aquaporin-2 apical sorting attrans-Golgi network in polarized MDCK cells (2009)
  • 著者名/発表者名: Yui N, Okutsu R, Sohara E, Rai T, OhtaA, Noda Y, Sasaki S, Uchida S
  • 雑誌名: Am. J. Physiol. Cell Physiol 巻: 297 ページ: 1389-1396
  • 査読あり
• [雑誌論文] Expression and phosphorylation of the Na+-Cl- cotransporter NCC in vivo isregulated by dietary salt, potassium, and SGK1 (2009)
  • 著者名/発表者名: Vallon V, Schroth J, Lang F, Kuhl D, Uchida S
  • 雑誌名: Am. J. Physiol. Renal Physiol 巻: 297 ページ: 704-12
  • 査読あり
• [雑誌論文] Function of aquaporin-7 in the kidney and the male reproductive system (2009)
  • 著者名/発表者名: Sohara E, Uchida S, Sasaki S
  • 雑誌名: Handb ExpPharmacol ページ: 219-31
  • 査読あり
• [雑誌論文] Dietary salt regulates the phosphorylation of OSR1/SPAK kinases and the sodium chloride cotransporter through aldosterone (2008)
  • 著者名/発表者名: Chiga M, Rai T, Yang SS, Ohta A, Takizawa T, Sasaki S, Uchida S
  • 雑誌名: Kidney Int 巻: 74 ページ: 1403-9
  • 査読あり
• [雑誌論文] Intracellular ClC-3 chloride channels promote bone resorption in vitro through organelle acidification in mouse osteoclasts (2008)
  • 著者名/発表者名: Okamoto F, Kajiya H, Toh K, Uchida S, Yoshikawa M, Sasaki S, Kido MA, Tanaka T, Okabe K
  • 雑誌名: Am. J .Physiol. Cell Physiol 巻: 294 ページ: C693-701
  • 査読あり
• [雑誌論文] AKAP220 colocalizes with AQP2 in the inner medullary collecting ducts (2008)
  • 著者名/発表者名: Okutsu R, Rai T, Kikuchi A, Ohno M, Uchida K, Sasaki S, Uchida S
  • 雑誌名: Kidney Int 巻: 74 ページ: 1429-33
  • 査読あり
• [学会発表] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates itsubiquitination (2012)
  • 著者名/発表者名: Hossain Khan MZ, Sohara E, Ohta A, Chiga M, Inoue Y, Isobe K, Wakabayashi M, Naito S, Oi K, Rai T, Sasaki S, Uchida S
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 20121100
• [学会発表] Development of sandwich enzyme linked immunosorbent assay (ELISA) for measurement of urinary total and phosphorylated Na-Clcotransporter (NCC) protein (2012)
  • 著者名/発表者名: Isobe K, Sohara E, Rai T, Sasaki S,Uchida S
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 20121100
• [学会発表] Chemical library screening for direct SPAK inhibitors by a newly developed ELISA system (2012)
  • 著者名/発表者名: Kikuchi E, Mori T, Isobe K, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 20121100
• [学会発表] Generation and analysis of WT-WNK4 transgenic mice revealed the physiological role of WNK4 (2012)
  • 著者名/発表者名: Mori T, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 20121100
• [学会発表] Hereditary nephrogenic diabetes insipidus (NDI) in Japanese patients: Analysis of 73 families (2012)
  • 著者名/発表者名: Sasaki S, Chiga M, Kikuchi E, Uchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 20121100
• [学会発表] WNK4-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone (2012)
  • 著者名/発表者名: Susa K, Sohara E, Isobe K, Chiga M, Rai T, Sasaki S, Uchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 20121100
• [学会発表] Dietary salt intake and angiotensin II regulates WNK-SPAK-NKCC1 phosphorylation cascade in mouse aorta (2012)
  • 著者名/発表者名: Zeniya M, Sohara E, Oi K, Chiga M, SusaK, Mori T, Rai T, Sasaki S, Uchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 20121100
• [学会発表] Generation and analyses of AQP11 BAC transgenic mice (2011)
  • 著者名/発表者名: Inoue Y, Sohara E, Kobayashi K, Rai T,Ishibashi K, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20111100
• [学会発表] Development of new systems to measure total and phosphorylated Na-Cl cotransporter(NCC)protein in human urine (2011)
  • 著者名/発表者名: Isobe K, Ohta A, Sohara E, Rai T, SasakiS, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20111100
• [学会発表] Urinary excretion of Na-ClCotransporter in exosomes is increased by high salt diet as well as low salt diet (2011)
  • 著者名/発表者名: Kahn MZH, Sohara E, Ohta A, Naito S, Chiga M, Rai T, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20111100
• [学会発表] CFTR is highly expressed in the cyst-lining epithelial cells of the AQP11 knockout mouse kidney (2011)
  • 著者名/発表者名: Kobayashi K, Uchida S, Sasaki S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20111100
• [学会発表] High throughput screening of drugs that inhibit WNK-OSR1/SPAK signaling cascade (2011)
  • 著者名/発表者名: Mori T, Ohta A, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20111100
• [学会発表] Increased Na-Cl cotransporter phosphorylation in hyperinsulinemic db/db mice is regulated by insulin/PI3K pathway (2011)
  • 著者名/発表者名: Nishida H, Sohara E, Alessi DR, Nomura N, Rai T, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20111100
• [学会発表] Chemical library screening for drugs to correct intracellular mislocalization of R8L mutant barttin (2011)
  • 著者名/発表者名: Nomura N, Naito S, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20111100
• [学会発表] Renal phenotype of WNK3 knockout mouse (2011)
  • 著者名/発表者名: Oi K, Sohara E, Rai T, Chiga M, Alessi DR, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20111100
• [学会発表] Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney (2011)
  • 著者名/発表者名: Sohara E, Rai T, Yang SS, Ohta A, Chiga M, Nomura N, Lin SH, Vandewalle A, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20111100
• [学会発表] WNK-OSR1/SPAK-SLC12A phosphorylation cascade in the WNK1 (+/-) mice (2011)
  • 著者名/発表者名: Susa K, Ohta A, Sohara E, Rai T, Kita S, Iwamoto T, Alessi DR, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society ofNephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20111100
• [学会発表] Increased protein abundance of the mutant WNK4 may be a cause of the increased WNK4 kinase activity inthe mouse model of pseudohypoaldosteronism type II (2011)
  • 著者名/発表者名: Wakabayashi M, Naito S, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20111100
• [学会発表] Treatment of Bartter syndrome type IV caused by R8L barttin mutation (2010)
  • 著者名/発表者名: Nomura N, Masato T, Ohta E, Ohta A, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 43nd Annual Meeting of American Society of Nephrology
  • 発表場所: Denver (USA)
  • 年月日: 20101100
• [学会発表] Investigation of the localization of mouse WNK3 in kidney (2009)
  • 著者名/発表者名: Oi K, Sohara E, Ohta A, Ohta E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 42nd Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 20091100
• [学会発表] Novel insulin-WNK4-NCC phosphorylation cascade is involved in pathogenesis of PHA II caused by WNK4R1185C mutation (2009)
  • 著者名/発表者名: Sohara E, Rai T, Ohta A, Ohta E, Sasaki S and Uchida S
  • 学会等名: The 42nd Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 20091100
• [学会発表] Increased phosphorylation of Na-Cl cotransporter (NCC) in the mouse model of pseudohypoaldosteronism type II was dependent on both OSR1 and SPAK kinase activity (2009)
  • 著者名/発表者名: Chiga M, Ohta A, Rai T, Sohara E, Ohta E, Rafiqi FH, Alessi DR, Sasaki S, Uchida S
  • 学会等名: The 42nd Annual Meeting ofAmerican Society of Nephrology
  • 発表場所: San Diego(USA)
  • 年月日: 20091000
• [学会発表] Regulation of WNK1 activation by extracellular ionic conditions (2009)
  • 著者名/発表者名: Naito S, Ohta A, Ohta E, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 42nd Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego(USA)
  • 年月日: 20091000
• [学会発表] Generation and analysis of the BsndR8L/R8L knockin mice (2009)
  • 著者名/発表者名: Nomura N, Masato T, Morimoto T, Kondo Y, Ohta E, Ohta A, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 42nd Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 20091000
• [学会発表] Effect of angiotensin II on WNK-OSR1/SPAK-NCC phosphorylation cascade in cultured mpkDCT cells and in vivo mouse kidney (2009)
  • 著者名/発表者名: Talati G, Ohta A, Rai T, Sohara E, Naito S, Vandewalle A, Sasaki S, Uchida S
  • 学会等名: The 42st Annual Meetingof American Society of Nephrology
  • 発表場所: SanDiego (USA)
  • 年月日: 20091000
• [学会発表] Molecular pathogenesis of Bartter syndrome caused by R8L barttin mutation (2009)
  • 著者名/発表者名: Uchida S, Nomura N, Sugawara N, Morimoto T, Kondo Y, Naito S, Yui N, Oi K, Talat G , KMZ Hossain, Wakabayashi M, Nishida H, Ohta E, Ohta A, Sohara E, Rai T, Sasaki S
  • 学会等名: The 40th NIPS International Symposium & Physiology of anion transport and cell volume regulation (PAT-CVR 2009)
  • 発表場所: Okazaki
  • 年月日: 20090800
• [学会発表] (2009)
  • 学会等名: The 36th International Congress ofPhysiological Sciences (IUPS2009)
  • 発表場所: Kyoto
  • 年月日: 20090700
• [学会発表] The primary cilia of the AQP11 knockout mouse kidney (2008)
  • 著者名/発表者名: Kobayashi K, Uchida S, Sasaki S
  • 学会等名: The 41st Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20081100
• [学会発表] Targeted disruption of the Wnk4 gene reduced the phospholylation of OSR1/SPAK and Na-Cl cotransporter in mice (2008)
  • 著者名/発表者名: Ohta A, Chiga M, Tajima M, Yang SS, Naito S, Rai T, Uchida S, Sasaki S
  • 学会等名: The 41st Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20081100
• [学会発表] Analysis of pancreatic exocrine function ofaquaporin-12 knockout mice (2008)
  • 著者名/発表者名: Ohta E, Itoh T, Nemoto T, Kumagai J, Ko SBH, Ishibashi K, Ohno M, Uchida K, Ohta A, Rai T, Uchida S, Sasaki S
  • 学会等名: The 41st Annual meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20081100
• [学会発表] Basic and Clinical Science Symposium, "Transport regulation by phosphorylation"Phosphorylation-dependent regulation of NCC by WNKs (2008)
  • 著者名/発表者名: Uchida S
  • 学会等名: The 41st Annual meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 20081100
• [学会発表] Epithelial transport: bridges between molecules and function
  • 著者名/発表者名: Uchida S
  • 学会等名: WNK kinases and cation-chloride cotransporters, novel transportsomes regulating blood pressure
• [備考] 東京医科歯科大学腎臓内科学
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm