尿路病原性大腸菌が産生するUSPの作用解析と粘膜ワクチンの開発

2010 年度 研究成果報告書

• 研究課題/領域番号: 20590436
• 研究種目: 基盤研究(C)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 細菌学(含真菌学)
• 研究機関: 帯広畜産大学
• 研究代表者: 倉園 久生 帯広畜産大学, 畜産学部, 教授 (90186487)
• 研究分担者: 山本 新吾 兵庫医科大学, 医学部, 教授 (80322741) ; 山崎 栄樹 帯広畜産大学, 畜産学部, 助教 (40514708)
• 研究期間 (年度): 2008 – 2010
• キーワード: 病原性 / 粘膜ワクチン

研究概要

尿路病原性大腸菌の産生する特異的な尿路病原因子であるUropathogenic-specific protein (USP)に対する高感度の検出系の構築を行なった。その結果、HRP-TMBZを用いたBead-ELISA系の感度が最も高く、その検出感度は100pg/mlであった。現在、この系を用いてUSPの産生量とUPECが惹起する病型との関係を検討すると共に、native USPの精製を行なっている。

研究成果

• [雑誌論文] Helicobacter pylori VacA reduces cellular expression of STAT3 and pro-survival Bcl-2 family proteins, Bcl-2 and Bcl-X_L, leading to apoptosis in gastric epithelial cells. (2011)
  • 著者名/発表者名: Matsumoto A, Kurazono H, Kohno S
  • 雑誌名: Digestive Diseases and Science 56 ページ: 999-1006
• [雑誌論文] Hybrid & El Tor variant biotypes of Vibrio cholerae Ol in Thailand (2011)
  • 著者名/発表者名: Na-Ubol M, Kurazono H, Chaicumpa W
  • 雑誌名: Indian J.Med.Res. 133 ページ: 387-394
• [雑誌論文] Double short-time exposure to pirarubicin produces higher cytotoxity against T24 bladder cancer cells. (2011)
  • 著者名/発表者名: Maruyama T, Yamamoto S, Shima H
  • 雑誌名: J.Infect.Chemother. 17 ページ: 11-16
• [雑誌論文] A single-dose regimen for antimicrobial prophylaxis to prevent perioperative infection in urological clean and clean-contaminated surgery. (2011)
  • 著者名/発表者名: Higuchi Y, Takesue Y, Yamamoto S
  • 雑誌名: J.Infect.Chemother. 17 ページ: 219-222
• [雑誌論文] A V_HH that neutralizes the zinc-metalloproteinase activity of botulinum neurotoxin type A. (2010)
  • 著者名/発表者名: Thanongsaksrikul J, Kurazono H, Chaicumpa W
  • 雑誌名: J.Biol.Chem. 285 ページ: 9657-9666
• [雑誌論文] Helicobacter pylori CagA Inhibits Endocytosis of Cytotoxin VacA in Host Cells. (2010)
  • 著者名/発表者名: Akada KJ, Kurazono H, Nakamura K
  • 雑誌名: Disease Models & Mechanisms 3 ページ: 605-617
• [雑誌論文] Far-infrared rays control prostate cancer cells in vitro and in vivo. (2010)
  • 著者名/発表者名: Shima H, Yamamoto S
  • 雑誌名: Cent.Eur.J.Biol. 5 ページ: 178-189
• [雑誌論文] Prognostic of Japanese metastatic renal cell carcinoma (mRCC) patients in the cytokine era-a cooperative group report of 1,463 mRCC patients. (2010)
  • 著者名/発表者名: Naito S, Yamamoto S, Tomita Y
  • 雑誌名: Eur.Urol. 57 ページ: 317-315
• [雑誌論文] A transient increase of calcineurin phosphatase activity in living-donor kidney transplant recipient with acute rejection. (2010)
  • 著者名/発表者名: Fukudo M, Yamamoto S, Inui K
  • 雑誌名: Drug Metab.Pharmacokinet 25 ページ: 411-417
• [雑誌論文] The antitumour effect of γδ T Cells is enhanced by valproic acid-induced up-regulation of NKG2D ligands. (2010)
  • 著者名/発表者名: Suzuki T, Terao S, Acharya B, Naoe M, Yamamoto S, Okamura H, Gotoh A
  • 雑誌名: Anticancer Res. 30 ページ: 4509-4514
• [雑誌論文] Direct binding of gangliosides to Helicobacter pylori vacuolating cytotoxin (VacA) neutralizes its toxin activity. (2010)
  • 著者名/発表者名: Wada A, Yamasaki E, Senba M
  • 雑誌名: Glycobiology 20 ページ: 668-678
• [雑誌論文] Helicobacter pylori VacA-induced inhibition of GSK3 through the PI3K/Akt signaling pathway. (2009)
  • 著者名/発表者名: Nakayama M, Yamasaki E, Kurazono H, Hirayama T
  • 雑誌名: J.Biol.Chem. 284 ページ: 1612-1619
• [雑誌論文] Genetic profiles of fluoroquinolone-resistant Escherichia coli isolated from cystitis : phylogeny, virulence factors, PAlusp-subtypes, and mutation patterns. (2009)
  • 著者名/発表者名: Takahashi A, Kurazono H, Yamamoto S
  • 雑誌名: J.Clin.Microbiol. 47 ページ: 791-795
• [雑誌論文] Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin. (2009)
  • 著者名/発表者名: Kulkeaw K, Kurazono H, Chaicumpa W
  • 雑誌名: J.Proteomics 72 ページ: 270-282
• [雑誌論文] Clinical efficacy of oral administration of 200 mg gatifloxacin once daily for 3 days for the treatment of patients with uncomplicated cystitis. (2009)
  • 著者名/発表者名: Yamamoto S
  • 雑誌名: J.Infect.Chemother. 15 ページ: 104-107
• [雑誌論文] Diagnostic accuracy of volume-rendered computed tomography movie and other computed tomography-based imaging methods in assessment of renal vascular anatomy for laparoscopic donor nephrectomy. (2009)
  • 著者名/発表者名: Yamamoto S
  • 雑誌名: Int.Urol.Nephrol. 41 ページ: 785-790
• [雑誌論文] Basic fibroblast growth factor causes urinary bladder overactivity trough gap junction generation in the smooth muscle. (2009)
  • 著者名/発表者名: Imamura M, Yamamoto S, Ogawa O
  • 雑誌名: Am.J.Pysiol.Renal Physiol. 297 ページ: F46-F54
• [雑誌論文] Molecular characterization of Helicobacter pylori VacA-induction of IL-8 in U937 cells reveals a prominent role for p38MAP kinase in ATF-2, CREB and NF K B activation. (2008)
  • 著者名/発表者名: Hisatsune J, Kurazono H, Yamasaki E
  • 雑誌名: J.Immunol. 180 ページ: 5017-5027
• [雑誌論文] Molecular typing of Vibrio cholerae O1 isolates from Thailand by pulsed-field gel electrophoresis. (2008)
  • 著者名/発表者名: Tapchaisri P, Kurazono H, Chaicumpa W
  • 雑誌名: J.Health Popul.Nutr. 26 ページ: 79-87
• [雑誌論文] 21-hydroxylase deficiency presenting as an adrenal incidentaloma : Endocrinological evaluation and mutational analysis. (2008)
  • 著者名/発表者名: Takahashi S, Yamamoto S
  • 雑誌名: Endocrinologist 18 ページ: 207-210
• [雑誌論文] Diagnostic efficacy of free to total ratio of prostate-specific antigen and prostate-specific antigen velocity, singly and in combination, in detecting prostate cancer in patients with total serum prostate-specific antigen between 4 and 10 ng/ml. (2008)
  • 著者名/発表者名: Yamamoto S
  • 雑誌名: Int.Urol.Nephrol. 40 ページ: 85-89
• [雑誌論文] Antibiotic prophylaxis for transrectal prostate biopsy : a prospective randomized study of tosufloxacin versus levofloxacin. (2008)
  • 著者名/発表者名: Yamamoto S
  • 雑誌名: Int J Urol 15 ページ: 604-606
• [雑誌論文] Salvage therapy trial for erectile dysfunction using phosphodiestrase type 5 inhibitors and vitamin E : Preliminary report. (2008)
  • 著者名/発表者名: Kondoh N, Yamamoto S
  • 雑誌名: Aging Male 11 ページ: 167-170
• [学会発表] on behalf of the Japanese UTI Research Group : A randomized clinical trial to evaluate the preventive effect of cranberry juice (UR65) for patients with recurrent urinary tract infection. (2011)
  • 著者名/発表者名: Takahashi S, Hamasuna R, Yasuda M, Arakawa S, Tanaka K, Ishikawa K, Kiyota H, Hayami H, Yamamoto S, Matsumoto T
  • 学会等名: ICC/ECCMID 2011
  • 発表場所: Milan, Italy
  • 年月日: 20110507-20110510
• [学会発表] Assessment of a single-dose regimen for antimicrobial prophylaxis to prevent perioperative infection in urologic surgery. (2010)
  • 著者名/発表者名: Yamamoto S
  • 学会等名: German-Japanese Confederation of Urology 9th Scientific Meeting.
  • 発表場所: Munster, Germany
  • 年月日: 20100619-20100626
• [学会発表] Enhancement of γδ T cell antitumor activity against prostate cancer cell through Toll-like receptor 2 and 3 ligand dependent activation. (2010)
  • 著者名/発表者名: Suzuki T, Tacharya B, Terao S, Noj ima M, Yamamoto S, Gotoh A
  • 学会等名: AUA Annual Meeting '10
  • 発表場所: San Francisco, USA
  • 年月日: 20100529-20100603
• [学会発表] 尿路病原性大腸菌の産生するUSPに対する特異的同定法の開発 (2010)
  • 著者名/発表者名: 山崎栄樹、高橋彰、山本新吾、星英之、牧野壮一、倉園久生
  • 学会等名: 第83回日本細菌学会総会
  • 発表場所: パシフィコ横浜、横浜市
  • 年月日: 20100327-20100329
• [学会発表] Analysis of Pthogenicity island of Uropathogenic Escherichia coli which encodes the USP. (2010)
  • 著者名/発表者名: 倉園久生倉園久生
  • 学会等名: SPU Forum : Updates on Common Viral and Bacterial Infections.
  • 発表場所: Universiti Malaysia Sabah, School of Medicine
  • 年月日: 2010-10-25
• [学会発表] Assessment of a single-dose regimen for antimicrobial prophylaxis to prevent perioperative infection in urologic surgery. (2010)
  • 著者名/発表者名: Higuchi Y, Yamada Y, Ueda Y, Suzuki Y, Aihara K, Maruyama T, Nojima M, Yamamoto S
  • 学会等名: Society for Infection and Inflammation (SIIU) at AUA Annual Meeting '10.
  • 発表場所: San Francisco, USA
  • 年月日: 2010-05-30
• [学会発表] Diversity in the expression of Salmonella enterotoxin that has a cytotoxic effect on cultured cells by various Salmonella species. (2009)
  • 著者名/発表者名: Yamasaki E, Nakano M, Hoshi H, Seto Y, Chongsa-nguan M, Chicumpa W, Nair G B, Makino S-I, Hirayama T, Kurazono H
  • 学会等名: 3rd ASM Conference on Salmonella : Biology, Pathogenesis & Prevention.
  • 発表場所: Aix-en-Provence, France
  • 年月日: 20091005-20091009
• [学会発表] Transient disappearance of donor-specific antibody on post-transplant day 1 may indicate high risk for antibody-mediated acute rejection following kidney transplantation in sensitized recipients. (2009)
  • 著者名/発表者名: Ueda Y, Nojima M, Hashimoto M, Higuchi Y, Kinoshita T, Hao H, Kyo M, Ichikawa Y, Hirota S, Yamamoto S, Shima H
  • 学会等名: European Society for Organ Transplantation 2009
  • 発表場所: Paris, France
  • 年月日: 20090827-20090830
• [学会発表] The phylogenetic and virulence profiles of quinolone-resistant Escherichia coli isolated from cystitis are different from quinolone-susceptible counterparts. (2009)
  • 著者名/発表者名: Yamamoto S, Takahashi A, Muratani T, Yasuda M, Takahashi S, Monden K, Ishikawa K, Kiyota H, Arakawa S, Matsumoto T, Shima H, Kurazono H
  • 学会等名: AUA Annual Meeting '09
  • 発表場所: Chicago, USA
  • 年月日: 20090426-20090430
• [学会発表] The antitumor effect of y6T cells against bladder cancer cells is enhanced following upregulation of NKG2D ligands by valproic acid. (2009)
  • 著者名/発表者名: Foru Suzuki, Bishnu Acharya, Shuji Terao, Shingo Yamamoto, Hiroki Shima, Haruki Okamura, Akinobu Gotoh
  • 学会等名: AUA Annual Meeting '09
  • 発表場所: Chicago, USA
  • 年月日: 20090426-20090430
• [学会発表] Biological activities of Salmonella enterotoxin. (2008)
  • 著者名/発表者名: Yamasaki E, Hoshi H, Seto Y, Chongsa-nguan M, Chicumpa W, G B Nair, Makino S-I, Hirayama T, Kurazono H
  • 学会等名: The 43rd Joint Conference US-Japan Cooperative Medical Science Program, Cholera Panel
  • 発表場所: Fukuoka, Japan
  • 年月日: 20081117-20081119
• [学会発表] AAUS Symposium : Catheter Associated UTI : CAUTI European and Asian guidelines. (2008)
  • 著者名/発表者名: Yamamoto S
  • 学会等名: Pre-congress meeting of the 5th Asian Association of UTI and STD
  • 発表場所: Taipei, Taiwan
  • 年月日: 2008-11-29
• [備考] ホームページ等