大脳皮質味覚野の神経回路基盤の解明

2011 年度 研究成果報告書

• 研究課題/領域番号: 20592188
• 研究種目: 基盤研究(C)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 機能系基礎歯科学
• 研究機関: 日本大学
• 研究代表者: 小林 真之 日本大学, 歯学部, 准教授 (00300830)
• 研究協力者: BUCKMASTER Paul 米国スタンフォード大学, 医学部, 准教授
• 研究期間 (年度): 2008 – 2011
• キーワード: 神経科学 / 生理学 / 解剖学 / 薬理学 / 歯学

研究概要

ラット大脳皮質島領野(味覚野)における抑制性ニューロンの分布様式を明らかにすると共に,興奮性ニューロンと抑制性ニューロンによって構成される局所神経回路のメカニズムおよびアドレナリンβ受容体,ムスカリン受容体, GABA_B受容体の各種アゴニストによる修飾作用を複数の細胞から同時にホールセル記録を行うことによって明らかにした。

研究成果

• [雑誌論文] Disruption of programmed masticatory movements in unilateral MPTP-treated monkeys as a model of jaw movement abnormality in Parkinson's disease (2012)
  • 著者名/発表者名: Adachi K, Kobayashi M, Kawasaki T, Yokoyama C, Waddington JL, Sakagami H, Onoe H, Koshikawa N
  • 雑誌名: J Neural Transmission 巻: (in press)
  • 査読あり
• [雑誌論文] Histaminergic effects on the frequency of repetitive spike firing in rat insular cortex (2012)
  • 著者名/発表者名: Takei H, Song L, Ebihara K, Shirakawa T, Koshikawa N, Kobayashi M
  • 雑誌名: Neurosci Lett 巻: (in press)
  • 査読あり
• [雑誌論文] Spatiotemporal profiles of transcallosal connections in rat insular cortex revealed by in vivo optical imaging (2012)
  • 著者名/発表者名: Fujita S, Kitayama T, Mizoguchi N, Oi Y, Koshikawa N, Kobayashi M
  • 雑誌名: Neuroscience 巻: 206 ページ: 201-211
  • 査読あり
• [雑誌論文] Kinetics of GABA_B autoreceptor-mediated suppression of GABA release in rat insular cortex (2012)
  • 著者名/発表者名: Kobayashi M, Takei H, Yamamoto K, Hatanaka H, Koshikawa N
  • 雑誌名: J Neurophysiol 巻: 107 ページ: 1431-1442
  • 査読あり
• [雑誌論文] D_2-like dopamine receptors differentially regulate unitary IPSCs depending on presynaptic GABAergic neuron subtypes in rat nucleus accumbens shell (2012)
  • 著者名/発表者名: Kohnomi S, Koshikawa N, Kobayashi M
  • 雑誌名: J Neurophysiol 巻: 107 ページ: 692-703
  • 査読あり
• [雑誌論文] Gustatory imagery reveals functional connectivity from the prefrontal to insular cortices traced with magnetoencephalography (2011)
  • 著者名/発表者名: Kobayashi M, Sasabe T, Shigihara Y, Tanaka M, Watanabe Y
  • 雑誌名: PLoS ONE 巻: 6 ページ: e21736
  • 査読あり
• [雑誌論文] Spatiotemporal dynamics of long-term potentiation in rat insular cortex revealed by optical imaging (2011)
  • 著者名/発表者名: Mizoguchi N, Fujita S, Koshikawa N, Kobayashi M
  • 雑誌名: Neurobiol Learn Mem 巻: 96 ページ: 468-478
  • 査読あり
• [雑誌論文] GABA_B receptors accentuate neural excitation contrast in rat insular cortex (2011)
  • 著者名/発表者名: Fujita S, Koshikawa N, Kobayashi M
  • 雑誌名: Neuroscience 巻: 199 ページ: 259-271
  • 査読あり
• [雑誌論文] Functional mapping of gustatory neurons in the insular cortex revealed by pERK-immunohistochemistry and in vivo optical imaging (2010)
  • 著者名/発表者名: Kobayashi M, Fujita S, Takei H, Song L, Chen S, Suzuki I, Yoshida A, Iwata K, Koshikawa N
  • 雑誌名: Synapse 巻: 64 ページ: 323-334
  • 査読あり
• [雑誌論文] Spatiotemporal dynamics of excitation in rat insular cortex : intrinsic cortico-cortical circuit regulates caudal-rostro excitatory propagation from the insular to frontal cortex (2010)
  • 著者名/発表者名: Fujita S, Adachi K, Koshikawa N, Kobayashi M
  • 雑誌名: Neuroscience 巻: 165 ページ: 278-292
  • 査読あり
• [雑誌論文] Pilocarpine-induced status epilepticus causes acute interneuron loss and hyper-excitatory propagation in rat insular cortex (2010)
  • 著者名/発表者名: Chen S, Fujita S, Koshikawa N, Kobayashi M
  • 雑誌名: Neuroscience 巻: 166 ページ: 341-353
  • 査読あり
• [雑誌論文] Assessment of jaw movements by magnetic sensor in relation to topographies of orofacial behaviour in freely moving rats : Studies with the dopamine D(1)-like receptor agonists SKF 83822 vs SKF 83959 (2010)
  • 著者名/発表者名: Fujita S, Kiguchi M, Kobayashi M, Kinsella A, Koshikawa N, Waddington JL
  • 雑誌名: Eur J Pharmacol 巻: 632 ページ: 39-44
  • 査読あり
• [雑誌論文] Involvement of NMDA receptors in the ventrolateral striatum of rats in apomorphine-induced jaw movements (2010)
  • 著者名/発表者名: Fujita S, Kiguchi M, Kobayashi M, Koshikawa N, Waddington JL
  • 雑誌名: Brain Res 巻: 1322 ページ: 30-37
  • 査読あり
• [雑誌論文] Orofacial movements in phospholipase C-related catalytically inactive protein(PRIP)-1/2 double knockout mice : effect of the GABAergic agent diazepam and the D1 dopamine receptor agonist SKF 83959 (2010)
  • 著者名/発表者名: Tomiyama K, Song L, Kobayashi M, Kinsella A, Kanematsu T, Hirata M, Koshikawa N, Waddington JL
  • 雑誌名: Synapse 巻: 64 ページ: 714-720
  • 査読あり
• [雑誌論文] Postsynaptic cell type-dependent cholinergic regulation of GABAergic synaptic transmission in rat insular cortex (2010)
  • 著者名/発表者名: Yamamoto K, Koyanagi Y, Koshikawa N, Kobayashi M
  • 雑誌名: J Neurophysiol 巻: 104 ページ: 1933-1945
  • 査読あり
• [雑誌論文] Presynaptic interneuron subtype-and age-dependent modulation of GABAergic synaptic transmission by β-adrenoceptors in rat insular cortex (2010)
  • 著者名/発表者名: Koyanagi Y, Yamamoto K, Oi Y, Koshikawa N, Kobayashi M
  • 雑誌名: J Neurophysiol 巻: 103 ページ: 2876-2888
  • 査読あり
• [雑誌論文] Insulin facilitates repetitive spike firing in rat insular cortex via phosphoinositide 3-kinase but not mitogen activated protein kinase cascade (2010)
  • 著者名/発表者名: Takei H, Fujita S, Shirakawa T, Koshikawa N, Kobayashi M
  • 雑誌名: Neuroscience 巻: 170 ページ: 1199-1208
  • 査読あり
• [雑誌論文] Pre-and postsynaptic modulation of glutamatergic synaptic transmission by activation ofα_1- and β-adrenoceptors in layer V pyramidal neurons of rat cerebral cortex (2009)
  • 著者名/発表者名: Kobayashi M, Kojima M, Koyanagi Y, Adachi K, Imamura K, Koshikawa N
  • 雑誌名: Synapse 巻: 63 ページ: 269-281
  • 査読あり
• [雑誌論文] Activation of α_1-adrenoceptors increases firing frequency through protein kinase C in pyramidal neurons of rat visual cortex (2008)
  • 著者名/発表者名: Kobayashi M, Sasabe T, Shiohama Y, Koshikawa N
  • 雑誌名: Neurosci Lett 巻: 430 ページ: 175-180
  • 査読あり
• [雑誌論文] Developmental profile of GABA_A-mediated synaptic transmission in pyramidal cells of the somatosensory cortex (2008)
  • 著者名/発表者名: Kobayashi M, Hamada T, Kogo M, Yanagawa Y, Obata K, Kang Y
  • 雑誌名: Eur J Neurosci 巻: 28 ページ: 849-861
  • 査読あり
• [学会発表] Responses of insular cortical neurons to orofacial noxious stimulation (2012)
  • 著者名/発表者名: Adachi K, Beppu Y, Koshikawa N, Sakagami H, Shimada J, Kobayashi M
  • 学会等名: 第85回日本薬理学会年会
  • 発表場所: 京都国際会議場,京都
  • 年月日: 2012-03-16
• [学会発表] Target-cell dependent synaptic modulation of inhibitory synapses by nitric oxide in rat insular cortex (2012)
  • 著者名/発表者名: Yamamoto K, Koshikawa N, Kobayashi M
  • 学会等名: 第85回日本薬理学会年会
  • 発表場所: 京都国際会議場,京都
  • 年月日: 2012-03-15
• [学会発表] Spatiotemporal profiles of long-term potentiation in rat insular cortex : an optical imaging study (2011)
  • 著者名/発表者名: Mizoguchi N, Fujita S, Koshikawa N, Kobayashi M
  • 学会等名: 41st Annual Meeting of Society for Neuroscience
  • 発表場所: Washington DC, U. S. A
  • 年月日: 20111112-16
• [学会発表] Postsynaptic cell type-dependent modulation of inhibitory synaptic transmission by nitric oxide in rat insular cortex (2011)
  • 著者名/発表者名: Yamamoto K, Koshikawa N, Kobayashi M
  • 学会等名: 41st Annual Meeting of Society for Neuroscience
  • 発表場所: Washington DC, U. S. A
  • 年月日: 20111112-16
• [学会発表] Physiological and morphological features of layer II/III pyramidal cells in rat insular cortex (2011)
  • 著者名/発表者名: Adachi K, Koshikawa N, Kobayashi M
  • 学会等名: 8th IBRO World Congress of Neuroscience
  • 発表場所: Florence, Italy
  • 年月日: 20110714-18
• [学会発表] Reciprocal actions of leptin on excitatory and inhibitory synaptic transmission between pyramidal and fast spiking cells in rat cerebral cortex (2011)
  • 著者名/発表者名: Takei, H, Shirakawa T, Koshikawa N, Kobayashi M
  • 学会等名: 8th IBRO World Congress of Neuroscience
  • 発表場所: Florence, Italy
  • 年月日: 20110714-18
• [学会発表] Insulin increases spike firing frequency via PI3-K but not MAPK cascade in rat cortical pyramidal cells (2011)
  • 著者名/発表者名: Takei H, Koshikawa N, Kobayashi M
  • 学会等名: 第84回日本薬理学会年会
  • 発表場所: パシフィコ横浜,神奈川
  • 年月日: 2011-03-23
• [学会発表] Pilocarpine-induced status epilepticus causes hyper-excitatory propagation and interneuron loss in rat agranular insular cortex (2010)
  • 著者名/発表者名: Fujita S, Chen S, Mizoguchi N, Koshikawa K, Kobayashi M
  • 学会等名: 40th Annual Meeting of Society for Neuroscience
  • 発表場所: San Diego, U. S. A
  • 年月日: 20101113-17
• [学会発表] β-Adrenoceptors differentially regulate inhibitory synaptic transmission depending on presynaptic interneuron subtype in rat cerebral cortex (2010)
  • 著者名/発表者名: Koyanagi Y, Oi Y, Koshikawa K, Kobayashi M
  • 学会等名: 40th Annual Meeting of Society for Neuroscience
  • 発表場所: San Diego, U. S. A
  • 年月日: 20101113-17
• [学会発表] Postsynaptic cell type-dependent cholinergic regulation of GABAergic synaptic transmission in rat insular cortex (2010)
  • 著者名/発表者名: Yamamoto K, Koshikawa K, Kobayashi M
  • 学会等名: 40th Annual Meeting of Society for Neuroscience
  • 発表場所: San Diego, U. S. A
  • 年月日: 20101113-17
• [学会発表] Inter-and intracolumnar desynchronization by presynaptic GABA_B inhibition in the rat barrel cortex (2010)
  • 著者名/発表者名: Saito M, Sato H, Toyoda H, Kobayashi M, Althof D, Kulik A, Shigemoto R, Kang Y
  • 学会等名: 40th Annual Meeting of Society for Neuroscience
  • 発表場所: San Diego, U. S. A
  • 年月日: 20101113-17
• [学会発表] Regulation of excitatory propagation by GABA(A) and GABA(B) receptors in the insular cortex (2010)
  • 著者名/発表者名: Fujita S, Mizoguchi N, Koshikawa N, Kobayashi M
  • 学会等名: 第83回日本薬理学会年会
  • 発表場所: 大阪国際会議場,大阪
  • 年月日: 20100316-18
• [学会発表] Activation of dopamine D2 receptors suppresses lateral inhibition between medium spiny neurons in the nucleus accumbens (2010)
  • 著者名/発表者名: Kohnomi S, Koshikawa N, Kobayashi M
  • 学会等名: 第83回日本薬理学会年会
  • 発表場所: 大阪国際会議場,大阪
  • 年月日: 20100316-18
• [学会発表] β-Adrenoceptors differentially modulate inhibitory synaptic transmission depending on presynaptic interneuron subtype (2010)
  • 著者名/発表者名: Kobayashi M, Koyanagi Y, Yamamoto K, Koshikawa N
  • 学会等名: Neuro2010
  • 発表場所: 神戸国際会議場,兵庫
  • 年月日: 2010-09-03
• [学会発表] Postsynaptic cell type-dependent cholinergic regulation of GABAergic synaptic transmission in rat insular cortex (2010)
  • 著者名/発表者名: Yamamoto K, Kobayashi M, Koshikawa N
  • 学会等名: Neuro2010
  • 発表場所: 神戸国際会議場,兵庫
  • 年月日: 2010-09-02
• [学会発表] Functional connectivity of rat insular cortex revealed by optical imaging technique (2009)
  • 著者名/発表者名: Fujita S, Oi Y, Koshikawa K, Kobayashi M
  • 学会等名: 39th Annual Meeting of Society for Neuroscience
  • 発表場所: Chicago, U. S. A
  • 年月日: 20091017-21
• [学会発表] Functional mapping of gustatory neurons in the insular cortex revealed by pERK-immunohistochemistry and in vivo optical imaging (2009)
  • 著者名/発表者名: Kobayashi M, Fujita S, Koshikawa K
  • 学会等名: 39th Annual Meeting of Society for Neuroscience
  • 発表場所: Chicago, U. S. A
  • 年月日: 20091017-21
• [学会発表] Physiological features of pyramidal cells in rat insular cortex revealed by in vivo whole-cell patch clamp recording (2009)
  • 著者名/発表者名: Adachi K, Fujita S, Sakagami H, Koshikawa N, Kobayashi M
  • 学会等名: IUPS 2009
  • 発表場所: Kyoto
  • 年月日: 20090727-0801
• [学会発表] Functional connectivity in rat insular cortex revealed by in vivo optical imaging (2009)
  • 著者名/発表者名: Fujita S, Adachi K, Koshikawa N, Kobayashi M
  • 学会等名: IUPS 2009
  • 発表場所: Kyoto
  • 年月日: 20090727-0801
• [学会発表] Differential modulation of glutamatergic and GABAergic synaptic transmission by activating alpha1-and beta-adrenoceptors in rat cerebral cortex (2008)
  • 著者名/発表者名: Koyanagi Y, Kobayashi M, Koshikawa N
  • 学会等名: 6th Forum of European Neuroscience
  • 発表場所: Geneva, Switzerland
  • 年月日: 20080712-16
• [図書] Macroscopic connection of rat insular cortex : anatomical bases underlying its physiological functions. In : Translating Mechanisms of Orofacial Neurological Disorder. (2011)
  • 著者名/発表者名: Kobayashi M
  • 総ページ数: 285-303
  • 出版者: London, Academic Press
• [備考]
  • URL: http://www.dent.nihon-u.ac.jp/pharm/index.htm