| 研究課題/領域番号 |
20F20392
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| 研究機関 | 東京農工大学 |
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研究代表者 |
小松 健 東京農工大学, (連合)農学研究科(研究院), 准教授 (60451837)
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| 研究分担者 |
HAMIM ISLAM 東京農工大学, (連合)農学研究科(研究院), 外国人特別研究員
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| 研究期間 (年度) |
2020-11-13 – 2023-03-31
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| キーワード | potexvirus / necrosis |
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| 研究実績の概要 |
We prepared inoculum of PlAMV by local lesion isolations and propagation in test host plants for the inoculation test in the next season. However, a local lesion isolate from ornamental lily plants \could not cause infection to leaves of cut flowers of ornamental lilies. In contrast, we successfully detected PlAMV by RT-qPCR from lily plants inoculated with bulk lily leaves showing symptoms. To analyze mechanisms underlying necrotic symptoms, we employed Nicotiana benthamiana and PlAMV pathosystem. In this pathosystem, helicase domain is a determinant of necrotic symptoms caused by PlAMV (Komatsu et al., 2011). We transiently expressed helicase domain of PlAMV by agroinfiltration and extracted total RNA from these leaf tissues at 2 days after infiltration for RNA-seq analysis.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Even though we need a start-up time of the researcher during COVID-19 situation, we could perform RNA-seq analysis and prepared for amplicon-sequencing.
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| 今後の研究の推進方策 |
We will analyse the data of RNA-seq analysis to see whether some transcriptome changes are observed in the necrosis caused by the helicase domain of PlAMV in Nicotiana benthamiana. In parallel, we will perform amplicon-sequencing of PlAMV replicase region RT-PCR-amplified from lily plants. By bioinformatic analyses of this amplicon-sequencing, we would like to identify what kind of variants in the lily plant enhance infectivity.
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