研究実績の概要 |
The proof of principle of an advanced new radical pair based microscopy technique, Pump Field Probe (PFP) microscopy that allows the direct detection of transient spin correlated radical pairs by fluorescence microscopy was robustly tested experimentally. Our previous work on magnetic field effects on autofluorescence in living cells demands new techniques to provide detailed spectroscopic and structural information on the radical pairs involved and no existing techniques can provide this information. This work is now being prepared for publication. An article was published on Bioarxiv which aimed to reproduce our magnetic field effects on cellular autofluorescence (due to its significance) but which was unable to do so. A detailed investigation of the techniques used was undertaken along with theoretical simulations which demonstrated that the irradiation conditions employed in the reproduction experiment were not suitable to observe the magnetic field dependence. This response was published on BioarXiv. Full model system testing of the multi-wavelength TOAD microscope was completed with improved signal-to-noise ratio achieved over the original single wavelength implementation. New Labview based software to control the instrument was completed and which allows automatic switching between probe laser wavelengths, automatic detector balancing and automatic bandpass filter switching. Theoretical simulations were conducted for zero field triplet eigenstates in multinuclear systems. An invited article on magnetic field effects in biology was written and published.
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