| 研究課題/領域番号 |
20J15123
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| 研究機関 | 京都大学 |
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研究代表者 |
Mu Huiying 京都大学, 工学研究科, 特別研究員(DC2)
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| 研究期間 (年度) |
2020-04-24 – 2022-03-31
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| キーワード | reaction-based probes |
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| 研究実績の概要 |
Huiying Mu’s research mainly focused on the design and synthesis of novel reaction-based functional probes for detecting the tiny changes in biological specimens, such as pH and reactive sulfur species (RSS) which related to a variety of human diseases. The prepared probes were applied to fluorescence and/or photoacoustic imging in living cells and tumor-bearing mice.
After one year's research, she provided important findings on the development of activatable chemical probes based on these projects which were published two papers in the journals of ACS sensors and Chemical Communications as the first author. Finally, she successfully finished the research in her doctoral course in Kyoto University and she received her PhD degree certificate at the end of Mar/2021.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Based on the research in profeesor's lab, a new water-soluble and tumor-targeting NIR probe has been develped for fluorescence and PA imaging in living cells and tumor-bearing mice. Furthermore, a new type of thiol-reactive fluorescent probes were developed for small-molecular thiol sensing and thiol-containing protein labeling. By rational probe design, various approaches have been successfully developed in term of (1) addressing the aggregation issue of pH-responsive cyanine dyes in aqueous solutions, (2) improvement of the stability and selectivity of thiol probes under basic conditions, and (3) improvement of the application performance of bioimaging in living cells and tumor-bearing mice which providing a possibility for the early diagnosis of target-related diseases.
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| 今後の研究の推進方策 |
Labile Fe(II) is an essential nutrient for life and can can contribute to both
cell initiaction and growth, especially in cancer cells. I will develope the turn-on and ratiometric sensors based on the N-O bond clevage of oxime ester by optimized its reactivity and selectivity with Fe(II). I will evaluate the selectivity, water solubility, reactivity, stability and detection limit of these two sensors having a ligand group for Fe in different conditions. The binding affinity of sensors with exogenous and endogenous Fe(II) after stimulated by specific enzyme in live cells will be evaluated.
At the same time, my research based on pH-responsive and tumor-targeting NIR cyanine probe for in vivo fluorescence and photoacoustic imaging will be continue.
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