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2022 年度 実績報告書

Investigation of the role of radial glia in regulating LysoPtdGlc required for nervous system development

研究課題

研究課題/領域番号 20K06884
研究機関京都大学

研究代表者

GUY ADAM・TSUDA  京都大学, 生命科学研究科, 准教授 (30634779)

研究期間 (年度) 2020-04-01 – 2023-03-31
キーワードLipid biology / Developmental biology / Neuroscience / Neurochemistry / Cell biology
研究実績の概要

In this project, I made the discovery of two isomers of the axon guidance cue lysophosphatidylglucoside (LysoPtdGlc) that activate the orphan receptor GPR55 in a potentially novel mechanism. I found that LysoPtdGlc can exist in two biologically active isomeric forms, R-LysoPtdGlc and S-LysoPtdGlc, that are R/S stereoisomers. Whilst both isomers activate GPR55, R-LysoPtdGlc activates Galpha13 downstream of GPR55, whilst S-LysoPtdGlc activates GalphaS, and they induce polar-opposite chemotropic responses of axon growth. I confirmed this using drugs that specifically inhibit components of adenylyl cyclase pathway, and by specifically blocking different Galpha subunit pathways with inhibitory peptides. In summary, I have discovered a potential ligand bias or functional selectivity of GPR55.

  • 研究成果

    (4件)

すべて 2023 2022

すべて 雑誌論文 (1件) (うち査読あり 1件、 オープンアクセス 1件) 学会発表 (3件) (うち国際学会 1件、 招待講演 1件)

  • [雑誌論文] Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells2023

    • 著者名/発表者名
      Nagasaki Shinji C.、Fukuda Tomonori D.、Yamada Mayumi、Suzuki Yusuke III、Kakutani Ryo、Guy Adam T.、Imayoshi Itaru
    • 雑誌名

      Cell Structure and Function

      巻: 48 ページ: 31~47

    • DOI

      10.1247/csf.22074

    • 査読あり / オープンアクセス
  • [学会発表] Functional analyses of lysophospholipid/GPR55 signalling in neuronal circuit development2023

    • 著者名/発表者名
      Adam T. Guy
    • 学会等名
      20th Joint Mini-Symposium in Cell and Molecular Biology, National Taiwan University
    • 招待講演
  • [学会発表] Radial Glial secreted phospholipase A2 group V enzyme modulates LysoPtdGlc/GPR55-mediated nociceptive axon guidance in spinal cord development2022

    • 著者名/発表者名
      Adam T. Guy
    • 学会等名
      Cold Spring Harbor Laboratory Meeting "Molecular Mechanisms of Neuronal Connectivity"
    • 国際学会
  • [学会発表] Secreted phospholipase A2 group V expressed by radial glia modulates LysoPtdGlc/GPR55-mediated nociceptive axon guidance in spinal cord development2022

    • 著者名/発表者名
      Adam T. Guy
    • 学会等名
      Japan Neuroscience Society Meeting "Neuro2022"

URL: 

公開日: 2023-12-25  

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