| 研究課題/領域番号 |
20K07771
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
DIMITROV DIMITAR 沖縄科学技術大学院大学, 細胞分子シナプス機能ユニット, 技術員 (70568865)
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| 研究期間 (年度) |
2020-04-01 – 2023-03-31
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| キーワード | Alzheimer's disease / Neurodome / Disease model |
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| 研究実績の概要 |
During the last year, several components and culture media were tested to increase the frequency of neurodome formation, as per the initial plan. Several media components and substrates were identified to promote neurodome formation. In addition, the cell condition inside the neurodomes were checked with apoptosis markers and were shown to be healthy as monolayer neurons without increase apoptosis. In addition, the neurodomes also were shown to contain a mix of neurons and glial cells, and this was shown to be important for neuronal survival.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The neurodome formation is rather consistent and the tests done until now show healthy and functional neurons.
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| 今後の研究の推進方策 |
In the upcoming year I plan to:
1. Test and choose one best condition for neurodome formation.
2. Test neuronal and synaptic functionality using electrophysiology.
3. Use ApoE4 to induce overexpression of Amyloid-beta, and test plaque formation and tau phosphorylation.
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| 次年度使用額が生じた理由 |
In the last fiscal year, some platicware that were planned for purchase (Ibidi culture dishes) were out of stock by the manufacturer so coud not purchase. Thus this fiscal year, the remaining amount will be used for these plasticware.
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