| 研究実績の概要 |
Using iPSC-derived human neuronal cultures, I attempt to modify the cultures so that more cell grow in controlled clustered "neurodome" organization. This is advantageous because beta-amyloid plaques can form in the semi-3D neurodome cultures, wehreas in monolayer cultures it would diffuse in the medium. Thus, the neurodome cultures represent a better model for Alzheimer's disease. In addition, I found that in the semi-3D neurodome structures, the neuronal cells are healthier and also have better synaptic networks.
Using such neurodome neuronal cultures, I used to indice Alzheimer's like pathology by adding tau re-formed fibrils. The first publication using such cultures is in the final stages of preparation.
In addition, I found several molecules that additionaly enhance the formation of neurodomes.
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