| 研究課題/領域番号 |
20K08327
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| 研究機関 | 金沢大学 |
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研究代表者 |
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| 研究分担者 |
関 晃裕 金沢大学, 医学系, 特任助教 (00733859)
酒井 佳夫 金沢大学, 医学系, 准教授 (80401925)
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| 研究期間 (年度) |
2020-04-01 – 2024-03-31
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| キーワード | NASH / uncultured ADSCs |
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| 研究実績の概要 |
NASH is a major cause of chronic liver disease, and effective treatment should be developed. Freshly isolated uncultured adipose tissue-derived stromal cells (u-ADSCs) are advantageous, they have pluripotent and immunomodulatory capabilities. We compared characteristics of u-ADSCs from wild mice and from mice affected by NASH. In the context of surface antigens and gene expression profile features, u-ADSCs from wild mice and u-ADSCs from NASH mice, fed with NASH diet for 4 weeks (NASH-4w) or 12 weeks (NASH-12w), did not show marked differences, except for u-ADSCs isolated from NASH-4w mice which showed a slight increase in frequency of CD11b, CD45 and CD44 surface markers, indicating a possible presence of WBCs, as well as an enhanced cell cycle related gene expression character.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Regarding the research progression in FY2020, all experiments and analyses proceeded steadily. We performed as planned, a full flow cytometry and DNA microarray analyses of uncultured adipose tissue-derived stromal cells (u-ADSCs) and of NASH mice-derived u-ADSCs; regarding the flow cytometry analysis we investigated the expression of the following surface antigens: CD11b, CD73, CD31, CD34, CD90, CD29. CD45, CD105, CD44, Ly6c and Gr-1.
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| 今後の研究の推進方策 |
Following the characterization of uncultured adipose tissue-derived stromal cells (u-ADSCs), we will proceed with the investigation of the effect of wild type u-ADSCs treatment of NASH-cirrhotic mice by evaluating the hepatic inflammatory cells infiltration as well as extent of fibrosis in livers. We are planning to evaluate the hepatic inflammatory cells infiltration using immunohistochemical and flow cytometry analyses, furthermore fibrosis quantification will be performed by staining liver tissue of mice treated with u-ADSCs.
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| 次年度使用額が生じた理由 |
A part of the funds were not used in FY2020 due to the Coronavirus pandemic, funds were not used for the planned travelling expenses and conference fees (national and international conferences). All other expenses in FY2020 proceeded as planned. In FY2021, as well, it is expected the use of funds as planned in the original research plan.
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