| 研究課題/領域番号 |
20K08832
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
アブケセーサ イマド 国立研究開発法人理化学研究所, 生命医科学研究センター, 上級研究員 (10749906)
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| 研究期間 (年度) |
2020-04-01 – 2023-03-31
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| キーワード | Mycetoma / Tropical disease / Galleria mellonella / Antifungal |
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| 研究実績の概要 |
1- In April 2021 we started task 1 (Determine pharmacokinetics) currently we are working on determining pharmacokinetics(PK) and pharmacodynamics (PD) of OSRAVUCONAZOLE (FRCZ) and ITRACONAZOLE (ITZ).We will determine the therapeutic efficacy of FRCZ, via the methodology we have used for ITZ in the past [PMID:28992315]. We will also determine a pharmacokinetic profile for FRCZ in hemolymph over time, as previously described for ITZ [PMID:28992315].
2- We finalized the sample design. Total samples size set to 155.
Total Time points=6 (0hours, 6 hours, 30 hours,54 hours,72 hours,168 hours). Sample type PBS (healthy) (n=30). Itraconazole treated healthy (n=25). Ravuconazole healthy(n=25).PBS infected (n=25). Itraconazole infected(n=25). Ravuconazole infected(n=25).
3- We developed genomic annotation hub database for Galleria Mellonella model and for the pathogen Madurella Mycetomatis. The two annotation hub packages will be released for public FY2021
4- We developed BSgenome data package (Software infrastructure for efficient representation of full genomes) Galleria Mellonella model and for the pathogen Madurella Mycetomatis. The BSgenome packages will be released for public FY2021
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
As many projects and activities, this project was severally impacted by COVID-19 pandemic. The extended lockdown in FY2020 slightly delayed the tasks planned for FY2020 (Labs closed). But we set recovery plan to recover the delayed tasks.
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| 今後の研究の推進方策 |
According to our research plan in FY 2020 we are supposed to perform the following two tasks
Task1 aim to determine the pharmacokinetic profile of Fosravuconazole and Itraconazole. Task2 aim to infect the larvae which include two subtasks. The first subtask to inoculate Galleria mellonella with Madurella mycetomatis fungal pathogen and treat the larvae on 4h 28h and 76h larvae with Fosravuconazole and Itraconazole or solvent and monitor for 10 days to determine the larval survival. Task3 In this task the plan to make research visit to Erasmus University medical center in the Netherlands to discuss about the project implementation and progress. Due to extended lockdown lab closed and we are not able to purchase the Galleria mellonella larvae, the above task delayed. In April 2021 we started task 1 and currently we are working on determining pharmacokinetics and pharmacodynamics of Fosravuconazole and Itraconazole. Task 2 Infect the larvae, This task planned to finish in FY2021. The larvae season will start soon in June 2021, by then we'll be able to purchase the Galleria mellonella larvae and start task2.
The research visit will be schedule after the travel restrictions relax in 2021 or 2022.
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| 次年度使用額が生じた理由 |
Due to the impact of the COVID-19 lockdown, we asked to Incurring FY2020 budget to FY2021. The Incurring will be used as planned in the grant as planned.
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