| 研究課題/領域番号 |
20K09898
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| 研究機関 | 松本歯科大学 |
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研究代表者 |
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| 研究分担者 |
安藤 宏 松本歯科大学, 歯学部, 准教授 (30312094)
北川 純一 松本歯科大学, 歯学部, 教授 (50373006)
海野 俊平 松本歯科大学, 歯学部, 講師 (80418920)
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| 研究期間 (年度) |
2020-04-01 – 2024-03-31
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| キーワード | Swallowing Reflex / TRPA1 / TRPV4 |
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| 研究実績の概要 |
In this project our main objective is to explore the contributions of TRPA1 and TRPV4 channels in triggering of the swallowing reflex. So far we observed TRPA1 expression mainly on thin nerve fibers and fibroblast-like cells in the sub-epithelial connective tissue layers of the superior laryngeal nerve (SLN)-innervated swallowing-related regions. In the nodose-petrosal-jugular ganglionic complex (NPJc), they were mainly localized on unmyelinated neurons. We also observed that topical application of a chemical agonist for TRPA1, allyl isothiocyanate (AITC), in the swallowing-related regions dose-dependently facilitated the triggering of swallowing reflex. Prior topical application of a antagonist for TRPA1 significantly reduced the AITC-induced swallowing reflex.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Our research is progressing smoothly as planned. In last fiscal year we conducted immunohistochemistry studies to understand the expression profile of TRPA1 channels in the SLN-innervated swallowing related-regions and in the NPJc and we successfully localized the channels in the regions and the ganglia. We also investigated whether activation of the channels by agonists can trigger swallowing reflex. We observed that a chemical agonist for TRPA1 can trigger swallowing reflex in a dose-dependent manner up to a certain concentration. We also checked whether antagonist for TRPA1 can reduce the agonist-induced swallowing reflexes. We observed that a TRPA1 antagonist significantly reduced the number of AITC-induced swallowing reflexes.
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| 今後の研究の推進方策 |
As a future plan we will conduct various experiments to understand the following research topics:
1. Whether cold stimuli activate TRPA1 channels present in the swallowing-related regions to trigger swallowing reflex or not.
2. Whether mechanical stimuli activate TRPA1 channels present in the swallowing-related regions to trigger swallowing reflex or not.
3. Expression profiles of TRPV4 channels in the swallowing-related regions and ganglia.
4. Whether TRPV4 is functional to trigger swallowing reflex.
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| 次年度使用額が生じた理由 |
Need to buy antagonists and antibodies. We plan to buy in next fiscal year and use them in research.
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