| 研究課題/領域番号 |
20K12701
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
KOTSIFAKI Domna 沖縄科学技術大学院大学, 量子技術のための光・物質相互作用ユニット, 客員研究員 (70834117)
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| 研究期間 (年度) |
2020-04-01 – 2023-03-31
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| キーワード | Plasmonics / Optical Tweesers / Raman Spectroscopy / Bacteria |
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| 研究実績の概要 |
In the second year, I have both investigated the role of SiC as substrate material as well as explored the best conditions to achieve single-bacterium trapping. Firstly, I have studied theoretically the enhancement of the electromagnetic field due to the presence of the SiC substrates and the temperature increase using Comsol Multiphysics software. I found that the theoretical temperature increase was only one degree/mW power. However, sticking effects of bacteria into the plasmonic nanostructures even with low incident trapping laser power were observed. A possible explanation for this discrepancy between theory and experiment is due to the adhesion layer that can absorb a greater fraction of the incoming infrared light leading to a significant increase in the local temperature. For this reason, a SiO2 substrate was employed to minimize the sticking effect and to trap effectively a single bacterium.
Using E. coli bacteria (Gram-negative), I have investigated the performance of the proposed platform by studying the survival and reproduction mechanism at the single-particle level as antibiotics have been added to the surrounding medium. I have noticed that the E. coli bacterium loses its mobility in a short time after the antibiotic is added. I have confirmed these experimental results by collecting the Raman signal of trapped single-bacterium using the plasmonic substrates. Despite the good performance of this platform for Gram-negative bacteria, its applicability to Gram-positive bacteria generates some obstacles that need more exploration.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Up now the progress of the project is general smooth. However, some modifications have been done in order to follow the requirements of the project. First, I have replaced the SiC with SiO2 substrates after extensively theoretical and experimental study. I have evaluated the platform using Gram-positive and negative bacteria. However, the results for the Gram-negative bacteria should be confirmed using other techniques.
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| 今後の研究の推進方策 |
My next plan focuses on studying the issues arising using Gram-negative bacteria, publishing the experimental results as well as writing the final report.
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| 次年度使用額が生じた理由 |
The pandemic creates some issues that delay a lot the purchase process of consumables
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