| 研究実績の概要 |
While studies revealing the molecular mechanisms of action of condensin I subunits have emerged, detailed mechanistic activity of condensin II subunits on the assembly of mitotic chromosomes remains largely unexplored. During the research period, recombinant condensin II wild-type and mutant complexes were successfully purified, and the overall roles of non-SMC subunits CAP-D3, CAP-G2, and CAP-H2 were determined. CAP-D3 and CAP-H2 are essential for both chromosome association and chromosome axis formation by the condensin II complex while CAP-G2 is non-essential. During the final fiscal year, regulatory roles of CAP-D3 C-terminal tail region were investigated to find that it has suppressive effects on condensin II through both G2-dependent and G2-independent mechanisms. These findings were published in a peer-reviewed manuscript in the academic journal eLife. Further analysis of the CAP-D3 C-tail has led to determine that mitotic phosphorylation within the region is important to reduce the suppressive effect by the C-tail on the complex. Overall, this study has revealed the molecular mechanisms of action of condensin II non-SMC subunits that fine-tune the complex in the assembly of mitotic chromosomes.
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