研究課題
In the final fiscal year, the effect of phase separation on conformation and dynamics of the repetitive domain and full length minispidroin NR6C and NR12C were investigated. The tyrosine residue of the repetitive domain was affected upon LLPS phenomenon. Then, we made a mutant on the repetitive domain which substituted the tyrosine with serine residue. We found a drastic effect on the mutant, where the oligomerization was suppressed at higher kPi concentration, suggesting the important role of tyrosine residues in promoting oligomerization upon LLPS phenomenon. Overall, upon phase separation at higher kPi concentration, spider silk protein forms oligomers. The repetitive domain is getting more rigid at higher kPi concentration. The C-terminal domain (CTD) and tyrosine from the repetitive domain are important as stickers which mediate the oligomerization on the LLPS formation. Both domains work synergistically to promote oligomerization upon liquid-liquid phase separation. This phenomenon mediates the self-assembly of soluble spider dragline silk protein into insoluble hierarchical fiber.
すべて 2023 2022
すべて 雑誌論文 (2件) (うち査読あり 2件) 学会発表 (3件) (うち招待講演 2件)
Biomacromolecules
巻: 24 ページ: 1604-1616
10.1021/acs.biomac.2c01344
Science advances
巻: 8 ページ: 6043
10.1126/sciadv.abo6043
