| 研究課題/領域番号 |
20K16236
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| 研究機関 | 長崎大学 |
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研究代表者 |
バヤルサイハン ガンチメグ 長崎大学, 医歯薬学総合研究科(医学系), 助教 (80841353)
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| 研究期間 (年度) |
2020-04-01 – 2024-03-31
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| キーワード | Interleukin 27 / malaria / macrophage |
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| 研究実績の概要 |
We reported that IL-27 is produced during acute phase of the infection with Plasmodium chabaudi (Pcc) but negatively regulates the immune responses during at the chronic phase of the infection. To determine cell types that produce IL-27, we generated IL-27 reporter IL27p28-Venus mice and monitored its expression. DC, macrophage, monocytes and NK cells were the main producers of IL-27 over the course of Pcc infection. We hypothesized that IL-27 produced by these cells have differential roles in the regulation of immune responses during Plasmodium infection. We generated mice lacking IL-27 in DCs or in macrophages and compared the response of these mice to the infection with Pcc. Mice lacking IL-27 in DCs showed delay in the clearance of parasitemia than that in control mice during the acute phase of the infection. The frequencies of activated CD4+ T cells (CD11ahiCD49dhi) and IFN-γ production in the spleen in response to malaria antigen were higher in mice lacking IL-27 in DCs than those in control mice during chronic phase of the infection. Malaria specific antibodies were also higher in mice lacking IL-27 in DCs than those in mice lacking IL-27 in macrophages. The results suggest that IL-27 procduced by DCs may preferentially suppresses development of antigen specific response during infection with Pcc. IL-27 in DC or macrophages play differential roles in the immune responses against Plasmodium infection.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We are summarizing initial findings and preparing for publication. In meantime we would like to check if IL-27 from different cells enhance secondary infection.
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| 今後の研究の推進方策 |
As stated above, we are summarizing initial results and preparing for publication. Further research will determine role of IL-27 produced by DCs or macrophages in secondary infection with plasmodium. To test this, groups of mice will be rechallenged with the heterologous parasite after complete clearance of the parasite. Mice will be monitored for parasitemia and disease progress after rechallenge. Expansion of memory cells in the spleen and their function will be checked after the rechallenge.Also we would like to check if IL-27 produced by DCs or macrophages affect on NK cell function. We will check NK cells activation and function upon infection with plasmodiun in different conditional knockout mice and compare the response.
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| 次年度使用額が生じた理由 |
One of our knockout mice were not breeding well, we had to wait long time for new born mice. I need to purchase fluorescent antibodies, disposables and need to maintain conditional knockout mice for my experiments. Fluorescent-labeled
antibodies will be used for flow cytometry and immunohistochemistry, these are the fundamental assays in my research. Purified mouse cytokines for ELISA will be purchased. Other reagents and chemicals required for immunological assays and cell cultures must be purchased. Disposable laboratory tools and plastic wares will be used often in daily research assays.
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