Real-time observation of native conformations and molecular behaviors of viral fusion proteins using high-speed atomic force microscopy (HS-AFM).

2023 年度 実績報告書

• 研究課題/領域番号: 20K16262
• 研究機関: 金沢大学
• 研究代表者: LIM KEE・SIANG 金沢大学, ナノ生命科学研究所, 特任助教 (60842987)
• 研究期間 (年度): 2020-04-01 – 2024-03-31
• キーワード: HS-AFM nanoimaging / viral fusion protein / Hemagglutinin / Spike protein / Influenza A / SARS-CoV-2

研究実績の概要

From 2020-2023, the applicant conducted HS-AFM nanoimaging to study viral fusion proteins like hemagglutinin (HA; Influenza A) and spike protein (S protein; SARS-CoV-2). The results indicate HS-AFM's capability in capturing nanoscale structural dynamics of these proteins, including fusogenic transitions of HA, S protein-ACE2 interactions, and more. These transient structures are challenging to observe with cryo-EM and X-ray crystallography. Additionally, the applicant investigated neutralizing antibody-S protein interactions. Overall, the applicant showcases HS-AFM's potential for studying viral fusion proteins and identifying potential antiviral targets.

研究成果

• [雑誌論文] Crafty mimicry grants nuclear pore entry to HIV (2024)
  • 著者名/発表者名: Lim Keesiang、Hazawa Masaharu、Wong Richard W.
  • 雑誌名: Cell Host & Microbe 巻: 32 ページ: 441～442
  • DOI: 10.1016/j.chom.2024.03.004
  • 査読あり / 国際共著
• [雑誌論文] Nanoscopic Elucidation of Spontaneous Self-Assembly of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Open Reading Frame 6 (ORF6) Protein (2023)
  • 著者名/発表者名: Nishide Goro、Lim Keesiang、Tamura Maiki、Kobayashi Akiko、Zhao Qingci、Hazawa Masaharu、Ando Toshio、Nishida Noritaka、Wong Richard W.
  • 雑誌名: The Journal of Physical Chemistry Letters 巻: 14 ページ: 8385～8396
  • DOI: 10.1021/acs.jpclett.3c01440
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] Nanoscopic Assessment of Anti-SARS-CoV-2 Spike Neutralizing Antibody Using High-Speed AFM (2023)
  • 著者名/発表者名: Lim Keesiang、Nishide Goro、Sajidah Elma Sakinatus、Yamano Tomoyoshi、Qiu Yujia、Yoshida Takeshi、Kobayashi Akiko、Hazawa Masaharu、Ando Toshio、Hanayama Rikinari、Wong Richard W.
  • 雑誌名: Nano Letters 巻: 23 ページ: 619～628
  • DOI: 10.1021/acs.nanolett.2c04270
  • 査読あり / オープンアクセス / 国際共著
• [学会発表] Nanoscopic Evaluation of Anti-SARS-CoV-2 Spike Neutralizing Antibody Using High-Speed AFM (2023)
  • 著者名/発表者名: Keesiang Lim
  • 学会等名: the 7th WPI NanoLSI Symposium
• [学会発表] Nanoscopic Evaluation of Anti-SARS-CoV-2 Spike Neutralizing Antibody Using High-Speed AFM. (2023)
  • 著者名/発表者名: Keesiang Lim
  • 学会等名: The 70th Annual Meeting of the Japanese Society for Virology
• [学会発表] Nanoscopic Evaluation of Anti-SARS-CoV-2 Spike Neutralizing Antibody Using High-Speed AFM (2023)
  • 著者名/発表者名: Keesiang Lim
  • 学会等名: The 23rd annual meeting of the Protein Science Society of Japan