| 研究実績の概要 |
In order to study the role of tRNA and tiRNA in glioma, I developed different transgenic glioma cell lines with knockdown or overexpression of tRNA modifying enzymes. I discovered that Alkbh1 (m1A demethylase and regulator of tRNA cleavage) influce glioma protein synthesis rate, senescence, proliferation and mitochondrial function. RNA sequencing revealed various gene regulatory networks that were differentially regulated in Alkbh1 overexpressing glioma cells.
Moreover, I continued the focus on tRNA cleavage and oxidative stress and discovered an angiogenin independent non-canonical cleavage pattern of tRNA cleavage in vivo and in various tumor cell lines that possibly regulate cellular oxidative response (Rashad et al, J Cell Phys, 2021)
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