| 研究課題/領域番号 |
20K18092
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| 研究機関 | 鳥取大学 |
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研究代表者 |
ツナピ パナイオタ 鳥取大学, 医学部附属病院, プロジェクト研究員 (50767431)
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| 研究期間 (年度) |
2020-04-01 – 2023-03-31
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| キーワード | male infertility / endocrine disruptor / sperm / testis / perfluorinated chemicals |
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| 研究実績の概要 |
The animal model of exposure to low dose of perfluorooctanesulfonic acid (PFOS) for the adult rats has been created. The rats received intraperitoneal injections of PFOS for 12 weeks. Their body weight was significantly decreased compared to the Control group.
The animal model of exposure to both low and high doses of PFOS for the pre-pubertal age rats(4 week-old) had as a result all the treated animals to die before completing the treatment period of 12 weeks. During their treatment period, their body weight was gradually decreasing day by day. Some of the animals had seizures and incidents similar to heart attack.
PFOS appears to have severe effects on the well-being of the animals.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
Perfluorooctanesulfonic acid (PFOS)was not available in Japan, therefore we had to wait for some time until delivered from abroad. Because of the corona virus pandemic there was a delay. Additionally, the doses of PFOS were lethal for the pre-pubertal age rat model, which had as a result to reconsider the doses of PFOS and recreate the model with lower doses. This is also another reason for the slight delay.
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| 今後の研究の推進方策 |
Mating studies are planned. Female rats will be evaluated for pregnancy, the number of pups per female rat delivered will be listed and the body weight of the pups will be recorded daily. After the mating studies, the reproductive organs of the male rats will be obtained under general anesthesia, as well as sperm will be aspirated from the epididymal cauda and sperm parameters will be evaluated. Blood and urine samples will also be collected for further analysis. The parameters will be measured as described in the protocol.
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| 次年度使用額が生じた理由 |
Because of the delay on the delivery of the chemicals required to create the animal model, the beginning of the study delayed. Therefore the animal study, due to unexpected death of the animals in the per-pubertal age groups has been transferred for the next fiscal year. The remaining amount of money needs to be transferred for the smooth process of the study.
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