研究課題/領域番号 |
20K18103
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研究機関 | 国際医療福祉大学 |
研究代表者 |
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研究期間 (年度) |
2020-04-01 – 2023-03-31
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キーワード | testis-specific lncRNAs / spermatogenesis / arsenic |
研究実績の概要 |
Testis-specific long non-protein coding RNAs (lncRNAs) are greatly focused as important regulators for mammalian spermatogenesis. Using IHC analysis, we found new testis-specific lncRNAs (1700101O22Rik and 1700108J01Rik), which are exclusively expressed in the mouse spermatogenetic cells. For functional study, we tried in vivo knockdown of these lncRNA using microinjection of retrovirus into the seminiferous tubules of mouse. We also tried in vitro functional analysis using mouse spermatogenetic cells (GC-1 cells). In vitro study, arsenic exposure of GC-1 cells showed down-regulation of 1700108J01Rik. Therefore, 1700108J01Rik may be an important target gene of arsenic and the downregulated expression of 1700108J01Rik may be closely related to male reproductive toxicity induced by arsenic.
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現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
Because of the pandemic outbreak of coronavirus, the performing of experiments were delayed than expected. In vivo functional analysis, our target cells (spermatogonia and spermatocytes) were not successfully transfected by retrovirus. The transfection mainly expressed in the sertoli cells, in which the target testis-specific lncRNAs were not positive. Currently, I am planning to perform RNAseq analysis in order to know the possible mechanisms or functions related to the target lncRNAs.
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今後の研究の推進方策 |
The main purpose of this study is to investigate the function of newly-identified testis-specific lncRNAs. From the in situ hybridization analysis, the target lncRNAs showed the stage-specific and spermatogenesis-related cells suggesting that these lncRNAs may involved in the mouse spermatogenesis. As a supplemental part, I also tried arsenic exposure to mouse spermatogenetic cell lines. As a future plan, I want to perform the RNA-sequence analysis of target gene knockdown and arsenic-exposed spermatogenetic cells to study the spermatogenetic related pathways and mechanisms.
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次年度使用額が生じた理由 |
I would like to perform RNA-seq analysis and investigate the possible target regulators for the target testis-specific lncRNAs in mouse spermatogenesis. As a future plan, I would like to continue the pathway analysis or new regulators related to male reproductive functions and infertility.
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