| 研究実績の概要 |
To clarify the role of horizontal cells (HCs) in photoreceptor synapse reformation after retinal organoid transplantation, tamoxifen-induced conditional HC labeling and ablation model has been established in retinal degeneration mouse background. Mouse retinal organoids were transplanted to the HC-ablated degenerating retinas, and multielectrode array recording was conducted to assess the host-graft reconstruction. The host ganglion cells showed increased light responses with better signal-to-noise ratio compared to the regular transplantation preparation without horizontal cell disturbance. De novo synapses between host bipolar cells and graft photoreceptors were observed with the graft horizontal cell processes invaginated in the horizontal cell ablated retinas. These results indicate that the removal of horizontal cells from the degenerated retinas prior to transplantation facilitates the functional synapse formation between host bipolar cells and graft photoreceptors. The residual horizontal cells may be incapable of forming new synapses and hence physically block the interaction of host bipolar cells and graft photoreceptors upon transplantation.
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