| 研究実績の概要 |
To date, we have isolated and investigated a total of sixteen compounds present in different alcoholic beverages. The beverages include beer, red and white wine, sake, shochu, and whiskey, of varying brands. Some of the compounds are present in the all the variations of one type of beverage, but not in others, indicating that these could be markers for specific types of alcoholic beverage. One example is hordenine, a compound found in all seven brands of beer tested, but not found in any other type of alcoholic beverage.
Since many of the compounds investigated are volatile, gas chromatography with mass spectrometry (GC-MS) appears to be the most effective analysis method. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was also investigated, but as many of the compounds are polar, it was difficult to analyze them without greatly adjusting our routine method. The GC-MS method is simple and rapid, and can be adapted to tandem mass spectrometry for more sensitivity and reduction in background noise. The use of tandem columns also reduces analysis times significantly, adding to the rapidity of the method.
The extraction method is also simple and rapid, involving small amounts of beverage and removal of interfering substances with the use of ion-exchange cartridges utilizing the pass-through method. Some compounds require derivatization to improve the chromatography, but this can be easily accomplished after other compounds have been analyzed, removing the need for double analyses.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The initial research plan involved the identification of volatile and non-volatile compounds present in alcoholic beverages and the comparison of analysis methods during the first year (2020). This phase is almost complete, and the next phase of optimizing the extraction methods and instrument conditions is well under way.
This work has led to two manuscripts in preparation for peer reviewed publication, and several conference presentations.
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| 今後の研究の推進方策 |
We plan to continue with the original research plan, investigating different extraction methods such as solid-phase microextraction (SPME) and other direct matrix methods. Instrument parameters such as different capillary columns at different lengths will be examined, as well as heating times and fiber types for SPME. We will also begin extraction of spiked human samples (urine, blood) to start the optimization of the analysis of these compounds from biological matrices.
Although the COVID pandemic is still affecting the ability to attend conferences, we will continue to present online if possible, and to work to publish the results we have collected thus far.
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| 次年度使用額が生じた理由 |
Because of the COVID-19 pandemic, much of the funds planned to use for business travel to conferences in Japan and overseas were not used. If international and domestic travel resumes after the pandemic subsides, the travel funds will be used as planned. If not, the funds will be reallocated to other products that are related to participating in online conferences (video and audio equipment), and for other purchases related to the research project (consumables, equipment, etc.).
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