| 研究課題/領域番号 |
20K18989
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| 研究機関 | 福岡大学 |
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研究代表者 |
ウォーターズ ブライアン 福岡大学, 医学部, 講師 (00609480)
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| 研究期間 (年度) |
2020-04-01 – 2024-03-31
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| キーワード | alcohol biomarker / white wine / malic acid derivative / GC-MS / forensic toxicology |
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| 研究実績の概要 |
In 2022, we focused on the analysis of a malic acid ester, which we had difficulty confirming the idenitity of because of a lack of a reference standard. This derivative was one of the compounds we focused on as a possible marker, particularly in white wine. We conducted a study where we analyzed urine samples from volunteers who consumed white wine, and we found that this derivative was present in most samples. In the coming year, we want to determine if the concentration of this derivative in urine correlates with the amount of white wine consumed. Also, is this derivative only present in white wine drinkers, or in other alcoholic beverages? Future research will explore its use of as a marker for white wine consumption and investigate its detection window.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
In the first phase (2020) of this research, we were able to isolate compounds from alcoholic beverages that could act as drinking markers. In the second year (2021), we were able to eliminate compounds that were complicated to analyze, those that did not stay in the body longer than ethanol, and those that were not unique to alcohol consumption. Ethyl glucoside was idenitfied as a possible marker for the consuption of sake, and 2 original articles were published. In 2022, we turned our focus to a derivative of malic acid that appears to be present in the urine of white wine drinkers. Focusing on this one compound helps us to reliably develop an extraction and analysis method. Hopefully this work will continue to yield useful presentations and published papers.
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| 今後の研究の推進方策 |
The project will continue with the optimization of the extraction and analysis methods for the malic acid derivative. Although no known reference standard is available, we can still optimize the extraction and analysis methods. This year we will look at alternate extraction methods like simplified liquid extraction (SLE) and QuEChERS. GC-MS(/MS) analysis and LC-MS/MS analysis will be using for analysis. Urine is the most efficient sample to use because of its ease of collection, but we hope to also expand to whole blood. Our hope to be able to make this a routine analysis that can any laboratory can easily add to their battery of tests. Now that COVID restrictions for conferences are loosening, we hope to be able to present and publish our results more readily.
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| 次年度使用額が生じた理由 |
次年度の使用が生じた主な理由は、新型コロナウイルスの感染拡大が続いたことで、多くの国際学会、国内学会、研究会等が中止や延期となったためである。また、参加できた学会や研究会は、オンラインでの開催であったため、当初予定していた旅費の予算を使用しなかった。
今後の使用計画としては、オンライン会議やワークショップに積極的に参加し、研究成果を発表する予定である。また、研究目標を推進し、研究室や設備を最新の状態に保つために、引き続き、物品等の購入費用としても使用する予定である。
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| 備考 |
We hope to continue studying possible alcohol drinking markers in future projects. Our focus is to help Japanese society by learning more about the effects of alcohol consuption in forensic medicine.
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