| 研究実績の概要 |
The applicant has developed a protocol for the generation of human corneal epithelial cells from human pluripotent stem cells (hPSCs) using a serum-free and xeno-free culture system supplemented with small chemicals. The applicant has succeeded in the generation of hPSC-derived corneal epithelial cells characterized with significant expression of the eye ectoderm developmental marker and the corneal epithelial maturation marker in comparison with a conventional human corneal epithelial cell line (HCE-T). Moreover, the applicant has optimized the necessary culturing conditions of hPSC-derived corneal epithelial cells before their seeding in a microfluidic device. Meanwhile, the applicant has improved the flow system for the generation of blinking-like stimulus using an air pumping system.
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| 次年度使用額が生じた理由 |
As the applicant has changed the research institution, consumables for the fabrication of microfluidic devices, culture media and reagents for the cultivation and differentiation of human pluripotent stem cells are to be included. Besides, due to the COVID-19 pandemic, the applicant could not use the budget for attending international or domestic meetings effectively. Moreover, as this is the last year of the project, the applicant plans to report the results in the form of a research paper based on the experimental results , so the costs associated with this are included. Therefore, the applicant is expecting to use the budget in the current fiscal year for the above-mentioned purposes.
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