| 研究実績の概要 |
In this project, a human corneal epithelium-on-a-chip (CEpOC) was created to test the efficacy and toxicity of ophthalmic drugs. The CEpOC was generated in microfluidic devices under eye-blinking-like stimuli. Moreover, metabolite transportation and secretion in CEpOC were assessed by untargeted metabolomic analysis, revealing biologically relevant metabolite changes, where a group of metabolites (e.g., glutathione and uric acid) were annotated as potential markers for cell toxicity. Furthermore, a simplified small molecule-based corneal induction method (SSM-CI) was developed to generate functional corneal epithelial cells from human pluripotent stem cells (hPSCs). This method resulted in mature corneal epithelial cells that have the potential to be used in CEpOC.
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