研究課題/領域番号 |
20K22659
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研究機関 | 熊本大学 |
研究代表者 |
Raja Erna 熊本大学, 国際先端医学研究機構, 特定事業研究員 (30770581)
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研究期間 (年度) |
2020-09-11 – 2022-03-31
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キーワード | stem cells / ECM / aging |
研究実績の概要 |
We are characterizing the aging process in skin inter-follicular epidermal stem cells consisting of the fast-dividing and slow-cycling stem cells. We found that the fast-dividing stem cells were gradually lost in aging, but slow-cycling stem cells were maintained.
Global gene expression analysis indicated that aging fast-dividing stem cells have altered cell-adhesion and extracellular matrix (ECM) components, one of which was Fibulin-7. Fibulin-7 knock-out led to early loss of fast-dividing stem cells and defective wound healing, suggesting impaired regenerative skin capacity.
Mechanistically, we propose that Fibulin-7 binds to other ECM proteins to promote ECM integrity and suppress stress signaling thus regulating the balance of stem cells renewal and differentiation.
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現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
In addition to reconfirmation and revision of previous data, we obtained some new data that could add further insight into the study. For example, differentiation changes during stem cells aging and also in the absence of Fibulin-7 in the skin stem cells extra-celullar matrix.
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今後の研究の推進方策 |
We plan to further examine the function of fibulin-7 in maintenance of ECM integrity and stem cells maintenance by proper renewal and differentiation process in the epidermis. We will attempt to identify which signaling pathway is relevant and where possible, to use small molecule inhibitors to promote or inhibit Fibulin-7 function in vitro and in vivo.
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次年度使用額が生じた理由 |
The remaining grant amount will be allocated for use in cell culture experiments, cell culture reagents, chemicals, antibodies, biochemical kits, and also animal husbandry costs.
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