| 研究課題/領域番号 |
20K22672
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
HUSNIK Filip 沖縄科学技術大学院大学, 進化・細胞・共生の生物学ユニット, 准教授 (30886130)
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| 研究期間 (年度) |
2020-09-11 – 2022-03-31
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| キーワード | symbiosis / genomics / insects / bacteria / microbiology |
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| 研究実績の概要 |
In the frame of the project, metagenomes of several mealybug species have been sequenced. Genomes of their endosymbiotic bacteria were closed into circular molecules, annotated, and analyzed with comparative genomics methods. Tissue samples from the same species were prepared for microscopy (FISH).
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
All samples submitted for genome sequencing worked on the first try and enough data was generated for all symbionts. My conservative plan was that some of the species might need optimization of protocols which tends to be time consuming, but no such troubleshooting was needed.
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| 今後の研究の推進方策 |
Additional samples will be processed and submitted for sequencing in mid-2021. Finishing all the genomics analyses sooner than planned will allow me to focus mostly on microscopic localization of the newly detected symbionts in the second year of the project.
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| 次年度使用額が生じた理由 |
Illumina sequencing costs are charged for a sequencing lane at our sequencing facility, so multiple samples have to be pooled together in order to fill the lane.
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