| 研究課題/領域番号 |
20K22969
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| 研究機関 | 筑波大学 |
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研究代表者 |
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| 研究期間 (年度) |
2020-09-11 – 2022-03-31
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| キーワード | newt / regeneration / scarless / physiology / tissue |
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| 研究実績の概要 |
To study tissue patterning in the regenerating newt the following transgenic animals were generated. 1 Transgenic Newts expressing the exogenous gene (fluorescent protein) in erythrocytes. To drive exogenous gene expression the mouse GATA1 erythroid enhancer was used. Expression was detected in circulating embryonic erythrocytes. 2 Transgenic Newts expressing the exogenous gene (fluorescent protein) for Shh sonic headhodge cell tracking. Both transgenic animals are now being growth into adult stages.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We used a previous transgenic protocol to develop the new Gata1 erythroid and Shh cell tracking transgenic system. Transgene plasmids were assemble during the summer 2020, and were available for transgenic microinjection by November 2020, in time for fertilized eggs collection of the newt. All resources for generation of transgenic animals for this were available, but some purchased items (reagents) were delayed due to impact of COVID on shipping.
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| 今後の研究の推進方策 |
GATA1 erythroid tracking animals will be validated for expression after metamorphosis. Also tissue and blood samples must be anazlyed with immunohistochemistry. After validation newtic1 shRNAi will used with this system to knockdown newtic1 in eyrthrocytes during limb regeneration. Shh tracking animals will be validated for expression after metamorphosis. Also tissue and blood samples must be anazlyed with immunohistochemistry using anti-shh antibody.
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| 次年度使用額が生じた理由 |
The reason for 44円 to be used for next fiscal year was due to research delay to order plastic cups for rearing GATA1 newt larvae. However research plan will contain because newt larvae are still developing and additional plastics cups are needed. Therefore, 44円 can be used for same research purpose.
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