膜電位イメージング法と生体染色を用いた胎生期脊髄内神経回路網の機能形成過程の追跡

2011 年度 研究成果報告書

• 研究課題/領域番号: 21500368
• 研究種目: 基盤研究(C)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 神経・筋肉生理学
• 研究機関: 駒沢女子大学
• 研究代表者: 佐藤 勝重 駒沢女子大学, 人間健康学部, 教授 (80291342)
• 連携研究者: 佐藤 容子 (SATO MOMOSE Yoko) 関東学院大学, 人間環境学部, 教授 (70251501)
• 研究期間 (年度): 2009 – 2011
• キーワード: 光学イメージング / 膜電位感受性色素 / 中枢神経系 / 機能発生 / embryo / 脊髄

研究概要

我々はこれまでに、個体発生過程の中枢神経系において、中枢神経系全体に広がる脱分極波が自発性に引き起こされることを見いだしてきた。本研究では、膜電位の光学的イメージング法を発生過程の鶏胚中枢神経系に適用して、その起源について解析を行った。その結果、自発性脱分極波はまず上部頸髄/下部延髄の限られた領域から発生し、個体発生が進むに従って、その起源が脊髄全体に広がっていくことを見いだした。このことから、神経の興奮性の時空間的違いが、脱分極波発現の背景にあることを示唆された。

研究成果

• [雑誌論文] Optical analysis of developmental changes in synaptic potentiation in the neonatal rat corticostriatal projection (2012)
  • 著者名/発表者名: Mullah, S. H.-E.-R., Inaji, M., Nariai, T., Momose-Sato, Y., Sato, K. and Ohno, K
  • 雑誌名: Neuroscience 巻: 201 ページ: 338-348
  • 査読あり
• [雑誌論文] Voltage-sensitive dye imaging analysis of functional development of the neonatal rat cortocostriatal projection (2011)
  • 著者名/発表者名: Inaji, M., Sato, K., Momose-Sato, Y. and Ohno, K
  • 雑誌名: NeuroImage 巻: 54 ページ: 1831-1839
  • 査読あり
• [雑誌論文] The embryonic brain and development of vagal pathways (2011)
  • 著者名/発表者名: Momose-Sato, Y. and Sato, K
  • 雑誌名: Respiratory Physiology and Neurobiology 巻: 178 ページ: 163-173
  • 査読あり
• [雑誌論文] Functional development of the vagal and glossopharyngeal nerve-related nuclei in the embryonic rat brainstem : Optical mapping with a voltage-sensitive dye (2011)
  • 著者名/発表者名: Momose-Sato, Y., Nakamori, T. and Sato, K
  • 雑誌名: Neuroscience 巻: 192 ページ: 781-792
  • 査読あり
• [雑誌論文] Demonstration of a neural circuit critical for imprinting behavior in chicks (2010)
  • 著者名/発表者名: Nakamori, T., Sato, K., Atoji, Y., Kanamatsu T., Tanaka, K. and Ohki-Hamazaki, H
  • 雑誌名: Journal of Neuroscience 巻: 30 ページ: 4467-4480
  • 査読あり
• [雑誌論文] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo (2009)
  • 著者名/発表者名: Mochida, H., Sato, K. and Momose-Sato, Y
  • 雑誌名: European Journal of Neuroscience 巻: 29 ページ: 14-30
  • 査読あり
• [雑誌論文] Spontaneous depolarization wave in the mouse embryo : origin and large-scale propagation over the CNS identified with voltage-sensitive dye imaging
  • 著者名/発表者名: Momose-Sato, Y., Nakamori, T. and Sato, K
  • 雑誌名: European Journal of Neuroscience 巻: (in press)
  • 査読あり
• [雑誌論文] Pharmacological mechanisms underlying the switching from the large-scale depolarization wave to segregated activity in the mouse CNS
  • 著者名/発表者名: Momose-Sato, Y., Nakamori, T. and Sato, K
  • 雑誌名: European Journal of Neuroscience 巻: (in press)
  • 査読あり
• [学会発表] Pharmacological nature of the depolarization wave in the mouse CNS-Dual switching and its developmental significance- (2012)
  • 著者名/発表者名: Momose-Sato, Y., Nakamori, T. and Sato, K
  • 年月日: 20120000
• [学会発表] Optical survey of polysynaptic pathway formation in the embryonic rat vagal system (2012)
  • 著者名/発表者名: Sato, K., Nakamori, T. and Momose-Sato, Y
  • 年月日: 20120000
• [学会発表] Dual switching in the pharmacological nature of the large-scale depolarization wave in the mouse embryo (2011)
  • 著者名/発表者名: Momose-Sato, Y., Nakamori, T. and Sato, K
  • 学会等名: Society for Neuroscience 41st Annual Meeting
  • 発表場所: Washington DC, USA
  • 年月日: 20111112-16
• [学会発表] Spontaneous depolarization wave in the mouse embryo : spatial segregation with development and its mechanism (2011)
  • 著者名/発表者名: Momose-Sato, Y. and Sato, K
  • 年月日: 20110000
• [学会発表] Screening of fluorescent voltagesensitive dyes for detecting neural activity in the embryonic brain (2011)
  • 著者名/発表者名: Sato, K., Komuro, R. and Momose-Sato, Y
  • 年月日: 20110000
• [学会発表] Spontaneous depolarization wave in the embryonic mouse CNS : disappearance of the wave with development and its mechanism (2011)
  • 著者名/発表者名: Momose-Sato, Y., Nakamori, T. and Sato, K
  • 年月日: 20110000
• [学会発表] Screening of voltage-sensitive fluorescence dyes for imaging embryonic neural activity (2011)
  • 著者名/発表者名: Sato, K., Komuro, R., Nakamori, T. and Momose-Sato, Y
  • 年月日: 20110000
• [学会発表] NR2B-dependent neural activation is indispensable for visual imprinting (2011)
  • 著者名/発表者名: Nakamori, T., Sato, K., Tanaka, K. and Ohki-Hamazaki, H
  • 年月日: 20110000
• [学会発表] Developmental changes in the spontaneous depolarization wave in the mouse embryo : mechanisms underlying spatial segregation (2010)
  • 著者名/発表者名: Momose-Sato, Y. and Sato, K.
  • 学会等名: Society for Neuroscience 40th Annual Meeting
  • 発表場所: San Diego, USA
  • 年月日: 20101113-17
• [学会発表] Functional distribution of the palm sensory area using intraoperative intrinsic optical imaging (2010)
  • 著者名/発表者名: Maehara, T., Inaji, M., Nariai, T., Sato, K. and Ohno, K
  • 学会等名: 29th International Congress of Clinical Neurophysiology
  • 発表場所: Kobe, Japan
  • 年月日: 20101028-1101
• [学会発表] Large-scale rhythmic depolarization wave in the embryonic avian and mammalian neural circuits (2010)
  • 著者名/発表者名: Sato, K. and Momose-Sato, Y
  • 年月日: 20100000
• [学会発表] Spontaneous depolarization waves in the developing mouse CNS : optical imaging with a voltage-sensitive dye (2010)
  • 著者名/発表者名: Momose-Sato, Y. and Sato, K
  • 年月日: 20100000
• [学会発表] Voltage-sensitive dye analysis of spontaneous depolarization waves in the developing mouse CNS (2010)
  • 著者名/発表者名: Momose-Sato, Y. and Sato, K
  • 年月日: 20100000
• [学会発表] Spontaneous depolarization waves in the mouse embryo revealed by optical imaging with a voltage-sensitive dye (2009)
  • 著者名/発表者名: Momose-Sato, Y. and Sato, K
  • 学会等名: Society for Neuroscience 39th Annual Meeting
  • 発表場所: Chicago, USA
  • 年月日: 20091017-21
• [学会発表] Functional development of auditory and vestibular neural circuits in the embryonic chick brainstem revealed by voltage-sensitive dye imaging (2009)
  • 著者名/発表者名: Sato, K., Glover, J. C. and Momose-Sato, Y
  • 学会等名: XXXVI International Congress of Physiological Sciences
  • 発表場所: Kyoto, Japan
  • 年月日: 20090727-0801
• [学会発表] Optical imaging of large-scale depolarization waves in the developing CNS (2009)
  • 著者名/発表者名: Momose-Sato, Y. and Sato, K
  • 学会等名: XXXVI International Congress of Physiological Sciences
  • 発表場所: Kyoto, Japan
  • 年月日: 20090727-0801
• [学会発表] A telencephalic neural circuit critical for visual imprinting in chicks (2009)
  • 著者名/発表者名: Nakamori, T., Sato, K., Tanaka, K. and Ohki-Hamazaki H
  • 学会等名: XXXVI International Congress of Physiological Sciences
  • 発表場所: Kyoto, Japan
  • 年月日: 20090727-0801
• [学会発表] Functional development of auditory and vestibular neural circuits in the embryonic chick brainstem revealed by voltage-sensitive dye imaging (2009)
  • 著者名/発表者名: Sato, K., Glover, J. C. and Momose-Sato, Y
  • 年月日: 20090000
• [学会発表] Optical imaging of large-scale depolarization waves in the developing CNS (2009)
  • 著者名/発表者名: Momose-Sato, Y. and Sato, K
  • 年月日: 20090000
• [学会発表] A telencephalic neural circuit critical for visual imprinting in chicks (2009)
  • 著者名/発表者名: Nakamori, T., Sato, K., Tanaka, K. and Ohki-Hamazaki, H
  • 年月日: 20090000
• [学会発表] Functional development of the N. IX and N. X-related nuclei in the embryonic rat brainstem : Optical mapping with voltage-sensitive dyes (2009)
  • 著者名/発表者名: Sato, K. and Momose-Sato, Y.
  • 年月日: 20090000
• [学会発表] Switching of the transmitters that mediate the depolarization wave in the embryonic chick CNS (2009)
  • 著者名/発表者名: Momose-Sato, Y., Mochida, H. and Sato, K
  • 年月日: 20090000
• [学会発表] Activation of a telencephalic circuit is critical for visual imprinting in chicks (2009)
  • 著者名/発表者名: Nakamori, T., Sato, K., Tanaka, K. and Ohki-Hamazaki, H
  • 年月日: 20090000
• [図書] Monitoring population membrane potential signals during functional development of neuronal circuits in vertebrate embryos. In : Membrane Potential Imaging in the Nervous System : Methods and Applications (2010)
  • 著者名/発表者名: Momose-Sato, Y., Sato, K. and Kamino, K.
  • 総ページ数: 83-96
  • 出版者: Springer-Verlag, New York
• [図書] Spreading synchronized activity in the embryonic brainstem and spinal cord. In : Comprehensive Developmental Neuroscience
  • 著者名/発表者名: Momose-Sato, Y. and Sato, K.
  • 出版者: Elsevier, USA(in press)
• [備考]
  • URL: http://square.umin.ac.jp/optical/