途上国に適用可能な硫黄サイクル微生物機能活性化・次世代水資源循環技術の創成

2013 年度 研究成果報告書

• 研究課題/領域番号: 21676004
• 研究種目: 若手研究(S)
• 配分区分: 補助金
• 研究分野: 土木環境システム
• 研究機関: 長岡技術科学大学
• 研究代表者: 山口 隆司 長岡技術科学大学, 工学研究科, 教授 (10280447)
• 研究協力者: クーシビライ パイラヤ タイ王国、コンケン大学, 講師
• 研究期間 (年度): 2009 – 2013
• キーワード: 下水 / 微生物 / 生物処理 / 環境浄化 / 微生物解析 / 硫黄動態

研究概要

都市下水処理の有機物除去の問題を解決する技術を開発するため、本研究では嫌気性汚泥床と下降流懸架式スポンジ槽とを組み合わせた水処理システムについて研究を行った。実証試験の結果、本システムにより浮遊生固形性物質と生物化学的酸素要求量の除去率は、夏季において95%以上、冬季において85%以上と高いレベルに達した。このことから本提案システムは顕在化している下水処理の問題を解決するひとつの方法になり得ることが分かった。加えて、嫌気的硫黄酸化反応が起こる嫌気性汚泥床について微生物解析したところ、Proteobacteria門に属する微生物が主要な微生物グループのひとつであることが明らかとなった。

研究成果

• [雑誌論文] Molecular characterization of anaerobic sulfur-oxidizing microbial communities in up-flow anaerobic sludge blanket reactor treating municipal sewage (2014)
  • 著者名/発表者名: A. A. Aida, M. Hatamoto, M.Yamamoto, S. Ono, A. Nakamura, M.Takahashi, T. Yamaguchi
  • 雑誌名: Journal of Bioscience and Bioengineering
• [雑誌論文] Microbial community composition of a down-flow hanging sponge (DHS) reactor combined with an up-flow anaerobic sludge blanket (UASB) reactor for the treatment of municipal sewage (2014)
  • 著者名/発表者名: K. Kubota, M. Hayashi, K. Matsunaga, A. Iguchi, A. Ohashi, Y. Li, T. Yamaguchi, H. Harada
  • 雑誌名: Bioresource Technology ページ: 144-150
• [雑誌論文] Characterization of the retained sludge in a down-flow hanging sponge (DHS) reactor with emphasis on its low excess sludge production (2014)
  • 著者名/発表者名: T. Onodera, K. Matsunaga, K. Kubota, R. Taniguchi, H. Harada, K. Syutsubo, T. Okubo, S. Uemura, N. Araki, M. Yamada, M. Yamauchi, T. Yamaguchi
  • 雑誌名: Bioresource Technology 巻: 136 ページ: 169-175
• [雑誌論文] Enhancement of denitrification in a down-flow hanging sponge reactor by effluent recirculation (2013)
  • 著者名/発表者名: N. Ikeda, T. Natori, T. Okubo, A. Sugo, M. Aoki, M. Kimura, T. Yamaguchi, H. Harada, A. Ohashi, S. Uemura
  • 雑誌名: Water Science and Technology 巻: 68(3) ページ: 591-598
• [雑誌論文] Energy saving system with high effluent quality for municipal sewage treatment by UASB-DHS (2012)
  • 著者名/発表者名: H. Tanaka, M. Takahashi, Y. Yoneyama, K. Syutsubo, K. Kato, A. Nagano, T. Yamaguchi, H. Harada
  • 雑誌名: Water Science and Technology 巻: 66(6) ページ: 1186-1194
• [雑誌論文] Detection of single-copy functional genes in prokaryotic cells by two-pass TSA-FISH with polynucleotide probes (2012)
  • 著者名/発表者名: S. Kawakami, T. Hasegawa, H. Imachi, T. Yamaguchi, H. Harada, A. Ohashi, and K. Kubota
  • 雑誌名: Journal of Microbiological Methods 巻: 88 ページ: 218-223
• [雑誌論文] The effect of salinity on nitrite accumulation in a DHS reactor (2012)
  • 著者名/発表者名: T. Natori, Y.Takemura, H. Harada, K. Abe, A. Ohashi, M. Kimura, T. Yamaguchi, T. Okubo, S. Uemura
  • 雑誌名: Journal of Chemical Technology and Biotechnology 巻: 87 ページ: 1466-1472
• [雑誌論文] Enrichment of anammox bacteria from mudflat sediments collected in Tokyo Bay (2012)
  • 著者名/発表者名: S. Uemura, N. Ikeda, T. Natori, T. Okubo, T. Yamaguchi, M. Kimura, A. Iguchi, H. Harada
  • 雑誌名: African Journal of Microbiology Research 巻: 6(16) ページ: 3778-3782
• [雑誌論文] Long term evaluation of the effect of salinity on organic removal and ammonium oxidation in a down-flow hanging sponge reactor (2012)
  • 著者名/発表者名: S. Uemura, M. Kimura, T. Yamaguchi, A. Ohashi, Y. Takemura, H. Harada
  • 雑誌名: International Journal of Environmental Research 巻: 6(2), Spring ページ: 361-366
• [雑誌論文] Methanolinea mesophila sp. nov., a hydrogenotrophic methanogen isolated from rice field soil, and proposal of the archaeal family Methanoregulaceae fam. nov. within the order Methanomicrobiales (2012)
  • 著者名/発表者名: S. Sakai, M. Ehara, 1.C. Tseng, T. Yamaguchi, S. L. Brauer, H. Cadillo-Quiroz, S. H. Zinder, H. Imachi
  • 雑誌名: International Journal of Environmental Research 巻: 62(6) ページ: 1389-1395
• [雑誌論文] Direct treatment of settled sewage by DHS reactors with different size sponge support media (2012)
  • 著者名/発表者名: S. Uemura, S. Suzuki, Y. Maruyama, A. Ohashi, T. Yamaguchi, H. Harada
  • 雑誌名: International Journal of Environmental Research 巻: 6(1) ページ: 25-32
• [雑誌論文] Cultivation of methanogenic community from subseafloor sediments using a continuous-flow bioreactor (2011)
  • 著者名/発表者名: H. Imachi, K. Aoi, E. Tasumi, Y. Saito, Y. Yamanaka, Y. Saito, T. Yamaguchi, H. Tomaru, R. Takeuchi, Y. Morono, F. Inagaki, K. Takai
  • 雑誌名: ISME J 巻: 5 ページ: 1913-1925
• [雑誌論文] Methanoregula formicica sp. nov., a methane-producing archaeon isolated from methanogenic sludge (2011)
  • 著者名/発表者名: Y. Tashiro, S. Sakai, M. Ehara, M. Miyazaki, T. Yamaguchi, H. Imachi
  • 雑誌名: International Journal of Environmental Research 巻: 61(1) ページ: 53-59
• [雑誌論文] Evaluation of sludge properties in a pilot scale UASB reactor for sewage treatment in temperate region (2011)
  • 著者名/発表者名: K. Syutsubo, W. Yoochatchaval, 1. Tsushima, N. Araki, K. Kubota, T. Onodera, M. Takahashi, T. Yamaguchi, Y. Yoneyama
  • 雑誌名: Water Science and Technology 巻: 64(10) ページ: 1959-1966
• [雑誌論文] Evaluation of treatment characteristics and sludge properties in a UASB reactor treating municipal sewage at ambient temperature (2011)
  • 著者名/発表者名: M. Takahashi, A. Ohya, S. Kawakami, Y. Yoneyama, T. Onodera, K. Syutsubo, S. Yamazaki, N. Araki, H. Harada, T. Yamaguchi
  • 雑誌名: International Journal of Environmental Research 巻: 5(4) ページ: 821-826
• [雑誌論文] Performance of a pilot-scale sewage treatment : An up-flow anaerobic sludge blanket (UASB) and a down-flow hanging sponge (DHS) reactors combined system by sulfur-redox reaction process under low-temperature conditions (2011)
  • 著者名/発表者名: M. Takahashi, T. Yamaguchi, Y. Kuramoto, A. Nagano, S. Shimozaki, H. Sumino, N. Araki, S. Yamazaki, S. Kawakami, H. Harada
  • 雑誌名: Bioresource Technology 巻: 102 ページ: 753-757
• [雑誌論文] Removal of organic substances and oxidation of ammonium nitrogen by a down-flow hanging sponge (DHS) reactor under high salinity conditions (2010)
  • 著者名/発表者名: S. Uemura, S. Suzuki, K. Abe, K. Kubota, T.Yamaguchi, A. Ohashi, Y. Takemura, H. Harada
  • 雑誌名: Bioresource Technology 巻: 101 ページ: 5180-5185
• [雑誌論文] Application of a Single-chamber Microbial Fuel Cell (MFC) for organic wastewater treatment : influence of changes in wastewater composition on the process performance (2010)
  • 著者名/発表者名: K. Kubota, W. Yoochatchaval, T. Yamaguchi, K. Syutsubo
  • 雑誌名: Sustainable Environment Research 巻: 6(20) ページ: 347-351
• [雑誌論文] Evaluation of sludge properties in a pilot scale UASB reactor for sewage treatment in temperate region (2010)
  • 著者名/発表者名: K. Syutsubo, W. Yoochatchaval, I. Tsushima, N. Araki, K. Kubota, T. Onodera, M. Takahashi, T. Yamaguchi, Y. Yoneyama
  • 雑誌名: 12th World. Congr. Anaerobic Dig 巻: 90
• [雑誌論文] Detection of denitrifying bacteria by tyramide signal amplification - fluorescence in situ hybridization (TSA-FISH) (2010)
  • 著者名/発表者名: M. Aoki, K. Maetani, N. Araki, S. Kawakami, T. Yamaguchi
  • 雑誌名: 17th Asian Symposium on Ecotechnology 巻: 26
• [雑誌論文] Development of 16S rRNA gene-targeted primers for detection of archaeal anaerobic methanotrophs (ANMEs) (2009)
  • 著者名/発表者名: A. Miyashita, H. Mochimaru, H. Kazawa, A. Ohashi, T. Yamaguchi, K. Horikoshi, T. Nunoura, K. Takai, H. Imachi
  • 雑誌名: FEMS Microbiology. Letter 巻: 297(1) ページ: 31-37
• [雑誌論文] In Situ Detection and Quantification of Uncultured Members of the Phylum Nitrospirae Abundant in Methanogenic Wastewater Treatment Systems (2009)
  • 著者名/発表者名: A. Iguchi, T. Terada, T. Narihiro, T. Yamaguchi, Y. Kamagata, Y. Sekiguchi
  • 雑誌名: Microbes and Environments 巻: 24(2) ページ: 97-104
• [雑誌論文] Methanofollis ethanolicus sp. nov.,an ethanol-utilizing methanogen isolated from a lotus field (2009)
  • 著者名/発表者名: H. Imachi, S. Sakai, H. Nagai, T. Yamaguchi, K. Takai
  • 雑誌名: International Journal of Environmental Research 巻: 59 ページ: 800-805
• [雑誌論文] Influence of sugar content of wastewater on the microbial characteristics of granular sludge developed at 20℃ in the anaerobic granular sludge bed reactor (2009)
  • 著者名/発表者名: W. Yoochatchaval, I. Tsushima, T. Yamaguchi, N. Araki, H. Sumino, A. Ohashi, H. Harada, K. Syutsubo
  • 雑誌名: Journal of Environmental Science and Health PART A (Toxic/Hazardous Substance Environmental Engineering) 巻: 44 ページ: 921-927
• [雑誌論文] H. Tanaka, M. Takahashi, Y. Yoneyama, K. Syutsubo, K. Kato, A. Nagano, T.Yamaguchi, H. Harada
  • 著者名/発表者名: Down-flow hanging sponge; energy saving;excess sludge;municipal sewage treatment; up-flow anaerobic sludge blanket
  • 雑誌名: Water Science and Technology
  • DOI: doi:10.2166/wst.2012.297.
• [学会発表] Detection of Archaea in an upflow anaerobic sludge blanket reactor by in situ hybridization chain reaction-fluorescence in situ hybridization (2013)
  • 著者名/発表者名: T. Yamaguchi, S. Kawakami, M. Hatamoto, M. Takahashi, K. Kubota, H. Imachi, N. Araki, T. Yamaguchi
  • 学会等名: The 13th World Congress on Anaerobic Digestion
  • 年月日: 20130000
• [学会発表] A novel detection method for visualizing environmental microbes with low rRNA content and low cell permeability by using in situ HCR-FISH (2013)
  • 著者名/発表者名: T. Yamaguchi, S. Kawakami, M. Hatamoto, M. Takahashi, K. Kubota, H. Imachi, N. Araki, T. Yamaguchi
  • 学会等名: Annual Conference of the Association for General and Applied Microbiology
  • 年月日: 20130000
• [学会発表] Molecular analysis of microbial communities involved in anaerobic sulphur-oxidation in UASB reactor treating municipal sewage (2013)
  • 著者名/発表者名: A. A. Azmi, M. Hatamoto, M. Yamamoto, S. Ono, M. Takahashi, T. Yamaguchi
  • 学会等名: 13th World Congress on Anaerobic Digestion
  • 年月日: 20130000
• [学会発表] Phylogenetic diversity analysis of relevant to anaerobic/anoxic wastewater treatment (2013)
  • 著者名/発表者名: K. Kuroda, M. Hatamoto, A. Nakamura, M. Yamauchi, M. Yanlada, T. Yamaguchi
  • 学会等名: The 3rd International Symposium on Technology for Sustainability, accepted
  • 年月日: 20130000
• [学会発表] An integrated system of Sedimentation-DHS-A2SBR for effective removal of organic matter and nutrients from municipal wastewater (2013)
  • 著者名/発表者名: N. Maharjan, K. Dehanla, K. Ohtsuki, Y. Saito, Y. Miyaoka, T. Tshering, A. Nakamura, M. Hatamoto, T. Yamaguchi
  • 学会等名: Water and Environment Technology Conference
  • 年月日: 20130000
• [学会発表] Assessment of combined system of UASB-DHS-A_2SBR for municipal sewage treatment (2013)
  • 著者名/発表者名: Namita M., K. DEHAMA, K. OHTSUKI, T. CHOSEI, K. SAKAMOTO, Y. SAITO, Y. MIYAOKA, T.TSHERING, A. NAKAMURA, M. HATAMOTO and T.Yamaguchi
  • 学会等名: The 3rd International Symposium on Technology for Sustainability
  • 年月日: 20130000
• [学会発表] In situ HCR-FISH : a new sensitive fluorescence in situ hybridization technique for detecting environmental microorganisms (2012)
  • 著者名/発表者名: T. Yamaguchi, S. Kawakami, M. Hatamoto, M. Takahashi, K. Kubota, H. Imachi, N. Araki, T. Yamaguchi
  • 学会等名: 14th International Symposium on Microbial Ecology
  • 年月日: 20120000
• [学会発表] Microbial communities involved in anaerobic sulfur-oxidation in UASB reactor treating municipal sewage (2012)
  • 著者名/発表者名: A. A. Azmi, M. Hatamoto, M. Yamamoto, S. Ono, A. Nakamura, M. Takahashi, T. Yamaguchi
  • 学会等名: The 4th IWA Asia-Pacific Young Water Professionals Conference
  • 年月日: 20120000
• [学会発表] Characterization of anaerobic sulfur-oxidizing microbial communities in a UASB reactor treating municipal sewage (2012)
  • 著者名/発表者名: A. A. Azmi, M. Hatamoto, S. Ono, A. Nakamura, M. Takahashi, T. Yamaguchi
  • 学会等名: Water and Environnmnt Technology Conference
  • 年月日: 20120000
• [図書] 総説 -嫌気性生物処理を中心に,排水・汚水処理技術集成vol.2、第2編第1章 処理技術研究の最前線の総説を執筆 (2013)
  • 著者名/発表者名: 山口隆司,幡本将史,中村明靖,多川正,山崎慎一
• [図書] 微生物燃料電池による廃水処理システム最前線(第1編、第2章、第2節) (2013)
  • 著者名/発表者名: 山口隆司、山口剛士、幡本将史、中村明靖、川上周司、久保田健吾
  • 出版者: NTS
• [図書] 水浄化技術の最新動向, 2章4節「次世代水資源循環技術」中のpp.121-131を担当) (2011)
  • 著者名/発表者名: 山口隆司,高橋優信,幡本将史,川上周司,久保田健吾,原田秀樹,山田真義,山内正仁,荒木信夫, 山崎慎一
  • 出版者: シーエムシー出版
• [備考]
  • URL: http://ecolab.nagaokaut.ac.jp/