ケモカインBRAKを指標とした頭頸部扁平上皮癌に対するテーラーメイド医療の開発

2010 年度 研究成果報告書

• 研究課題/領域番号: 21792037
• 研究種目: 若手研究(B)
• 配分区分: 補助金
• 研究分野: 外科系歯学
• 研究機関: 神奈川歯科大学
• 研究代表者: 小澤 重幸 神奈川歯科大学, 歯学部, 助教 (40434394)
• 研究期間 (年度): 2009 – 2010
• キーワード: ケモカイン / BRAK / 扁平上皮癌

研究概要

BRAKは頭頸部扁平上皮癌で発現が低下もしくは消失している遺伝子である。私はBRAKの発現消失にプロモーター領域のメチル化が関与していることを明らかにした。

研究成果

• [雑誌論文] Reactive oxygen species (ROS) reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells. (2010)
  • 著者名/発表者名: Maehata Y, Ozawa S, Kobayashi K, Kato Y, Yoshino F, Miyamoto C, Izukuri K, Kubota E, Hata R, Lee MC.
  • 雑誌名: Free Radio Res 44(8) ページ: 913-924
  • 査読あり
• [雑誌論文] Chemokine CXCL14/BRAK transgenic mice suppress growth of carcinoma cell transplants. (2010)
  • 著者名/発表者名: Izukuri K, Suzuki K, Yajima N, Ozawa S, Ito S, Kubota E, Hata RI.
  • 雑誌名: Transgenic Res
  • 査読あり
• [雑誌論文] Expression of a chemokine BRAK/CXCL14 in oral floor carcinoma cells reduces the settlement rate of the cells and suppresses their proliferation in vivo. (2010)
  • 著者名/発表者名: Ito S, Ozawa S, Ikoma T, Yajima N, Kiyono T, Hata R.
  • 雑誌名: Biomed Res. 31(3) ページ: 199-206
  • 査読あり
• [雑誌論文] Human p38 delta MAP kinase mediates UV irradiation induced up-regulation of the gene expression of chemokine BRAK/CXCL14. (2010)
  • 著者名/発表者名: Ozawa S, Ito S, Kato Y, Kubota E, Hata R.
  • 雑誌名: Biochem Biophys Res Commun. 396(4) ページ: 1060-1064
  • 査読あり
• [雑誌論文] Functional characterization of proximal promoter of gene for human BRAK/CXCL14, a tumor-suppressing chemokine. (2010)
  • 著者名/発表者名: Komori R, Ozawa S, Kato Y, Shinji H, Kimoto S, Hata R.
  • 雑誌名: Biomed Res. 31(2) ページ: 123-131
  • 査読あり
• [雑誌論文] Expression of tumour-suppressing chemokine BRAK/CXCL14 reduces cell migration rate of HSC-3 tongue carcinoma cells and stimulates attachment to collagen and formation of elongated focal adhesions in vitro. (2010)
  • 著者名/発表者名: Sato K, Ozawa S, Izukuri K, Kato Y, Hata R.
  • 雑誌名: Cell Biol Int. 34(5) ページ: 513-522
  • 査読あり
• [雑誌論文] BRAK/CXCL14 expression in oral carcinoma cells completely suppresses tumor cell xenografts in SCID mouse. (2009)
  • 著者名/発表者名: Ozawa S, Kato Y, Kubota E, Hata R.
  • 雑誌名: Biomed Res. 30(5) ページ: 315-318
  • 査読あり
• [雑誌論文] Restoration of BRAK / CXCL14 gene expression by gefitinib is associated with antitumor efficacy of the drug in head and neck squamous cell carcinoma. (2009)
  • 著者名/発表者名: Ozawa S, Kato Y, Ito S, Komori R, Shiiki N, Tsukinoki K, Ozono S, Maehata Y, Taguchi T, Imagawa-Ishiguro Y, Tsukuda M, Kubota E, Hata R.
  • 雑誌名: Cancer Sol. 100(11) ページ: 2202-2209
  • 査読あり
• [学会発表] Oxidative stress reduce expression of CXCL14/BRAK in human cells (2011)
  • 著者名/発表者名: MAEHATA Y, OZAWA S, MIYAMOTO C, KOBAYASHI K, KOMORI R, HATA R.-I, LEE M.C
  • 学会等名: IADR General Session
  • 発表場所: San Diego
  • 年月日: 20110316-20110319
• [学会発表] Analysis of promoter region of huma BRAK/CXCL14 in SCC cells. (2011)
  • 著者名/発表者名: KOMORI R, OZAWA S, KATO Y, MAEHATA Y, SHINJI H, HATA R.-I, KIMOTO S
  • 学会等名: IADR General Session
  • 発表場所: San Diego
  • 年月日: 20110316-20110319
• [学会発表] Gene expression signatures at acidic extracellular pH in metastatic melanoma cells. (2010)
  • 著者名/発表者名: Kato Y, Ozawa S, Maehata Y, Maeda T, Kubota E, Tsukuda M, Miyazaki K, Hata R
  • 学会等名: Joint MRS-AACR Conference. Metastasis and the Tumor Microenvironment
  • 発表場所: Philadelphia
  • 年月日: 20100912-20100915
• [学会発表] Chemokine CXCL14/BRAK transgenic mice suppress growth and metastasis of carcinoma cells. (2010)
  • 著者名/発表者名: Hata R, Izukuri K, Suzuki K, Kato Y, Yajima N, Ozawa S, Ito S, Kubota E
  • 学会等名: 14th International Congress of Immunology
  • 発表場所: Kobe
  • 年月日: 2010-08-23
• [学会発表] Chemokine BRAK/CXCL14 is a multifunctional tumor suppressor. (2010)
  • 著者名/発表者名: Hata R, Izukuri K, Suzuki K, Yajima N, Ozawa S, Ito S, Kubota E
  • 学会等名: 88^<th> General Session & Exhibition of the IADR・5^<th> General Session of the Pan European Region of the IADR
  • 発表場所: Barcelona
  • 年月日: 2010-07-14
• [学会発表] BRAK/CXCL14 transgenic mice suppress growth of tumor cell xenografts. (2009)
  • 著者名/発表者名: Ryu-Ichiro Hata, Kazuhito Izukuri, Kenji Suzuki, Nobuyuki Yajima, Shin Ito, Shigeyuki Ozawa, Eiro Kubota
  • 発表場所: Boston
  • 年月日: 20091115-20091119
• [学会発表] Expression of BRAK/CXCL14 is associated with antitumor efficacy of gefitinib in head and neck squamous cell carcinoma (2009)
  • 著者名/発表者名: Ryu-Ichiro Hata, Shigeyuki Ozawa, Yasumasa Kato, Shin Ito, Reika Komori, Naoto Shiiki, Keiichi Tsukinoki, Yojiro Maehata, Eiro Kubota
  • 学会等名: ECCO15 and 34^<th> ESMO Multidisciplinary Congress
  • 発表場所: Berlin
  • 年月日: 20090920-20090924
• [学会発表] Functional Analysis of Promoter of Human BRAK/CXCL14, A Tumor Suppressor. (2009)
  • 著者名/発表者名: Reika Komori, Shigeyuki Ozawa, Yasumasa Kato, Hisaaki Shinji, Shigenari Kimoto, Ryu-Ichiro Hata
  • 学会等名: 87^<th> General Session & Exhibition of the IADR・38^<th> Annual Meeting of the AADR・33^<rd> Annual Meeting of the CADR
  • 発表場所: Miami Beach Convention Center, Miami, Florida
  • 年月日: 20090401-20090404
• [学会発表] A new mechanism of tumor suppression by gefitinib in HNSCC. (2009)
  • 著者名/発表者名: Shigeyuki Ozawa, Yasumasa Kato, Shin Ito, Reika Komori, Naoto Shiiki, Keiichi Tukinoki, Satoru Ozono, Yojiro Maehata, Takahide Taguchi, Yukari Imagawa-Ishiguro, Mamoru Tsukuda, Eiro Kubota, Ryu-Ichiro Hata
  • 学会等名: 87^<th> General Session & Exhibition of the IADR・38^<th> Annual Meeting of the AADR・33^<rd> Annual Meeting of the CADR
  • 発表場所: Miami Beach Convention Center, Miami, Florida
  • 年月日: 20090401-20090404
• [学会発表] Suppression of growth of Lewis lung carcinoma cell xenografts in BRAK transgenic mouse (2009)
  • 著者名/発表者名: Kazuhito Izukuri, Kenji Suzuki, Shigeyuki Ozawa, Eiro Kubota, Ryu-Ichiro Hata
  • 学会等名: Production of cancer resistant mouse. Yokosuka Science Festa
  • 発表場所: Shonan Village Center, Hayama
  • 年月日: 2009-07-06
• [学会発表] Chemokine BRAK stimulates apoptosis elicited by gefitinib in oral squamous cell carcinoma (2009)
  • 著者名/発表者名: Shin Ito, Shigeyuki Ozawa, Naoto Shiiki, Keiichi Tsukinoki, Eiro Kubota, Yasumasa Kato, Takahide Taguchi, Yukari Imagawa-Ishiguro, Mamoru Tsukuda, Ryu-Ichiro Hata
  • 学会等名: Yokosuka Science Festa
  • 発表場所: Shonan Village Center, Hayama
  • 年月日: 2009-07-06
• [学会発表] Expression of BRAK/CXCL14 is associated with antitumor efficacy of gefitinib in head and neck squamous cell carcinoma. (2009)
  • 著者名/発表者名: Shigeyuki Ozawa, Yasumasa Kato, Shin Ito, Reika Komori, Kenji Suzuki, Keiichi Tsukinoki, Yojiro Maehata, Takahide Taguchi, Yukari Imagawa-Ishiguro, Mamoru Tsukuda, Eiro Kubota, Ryu-Ichiro Hata
  • 学会等名: Yokosuka Science Festa
  • 発表場所: Shonan Village Center, Hayama
  • 年月日: 2009-07-06
• [学会発表] Functional analysis of promoter region of humanBRAK/CXCL14 (2009)
  • 著者名/発表者名: Reika Komori, Shigeyuki Ozawa, Yasumasa Kato, Hisaaki Shinji, Shigenari Kimoto, Ryu-Ichiro Hata
  • 学会等名: a tumor progression suppressor. Yokosuka Science Festa
  • 発表場所: Shonan Village Center, Hayama
  • 年月日: 2009-07-06
• [学会発表] Reactive oxygen species reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells. (2009)
  • 著者名/発表者名: Yojiro Maehata, Shigeyuki Ozawa, Chihiro Miyamoto, Kyo Kobayashi, Yasumasa Kato, Fumihiko Yoshino, Masaichi-Chang-il Lee, Ryu-Ichirc Hata
  • 学会等名: Yokosuka Science Festa
  • 発表場所: Shonan Village Center, Hayama
  • 年月日: 2009-07-05