| 研究課題/領域番号 |
21F20775
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
HUSNIK Filip 沖縄科学技術大学院大学, 進化・細胞・共生の生物学ユニット, 准教授 (30886130)
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| 研究分担者 |
DUNPHY COURTNEY 沖縄科学技術大学院大学, 進化・細胞・共生の生物学ユニット, 外国人特別研究員
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| 研究期間 (年度) |
2021-04-28 – 2023-03-31
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| キーワード | coral microbiome / functional dynamics / host microbiome / stability / ecosystem function |
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| 研究実績の概要 |
This past year, I was able to conduct multiple fieldwork expeditions and accomplish a large experiment and subsequent sample processing. Surveys were conducted along the west coast of Okinawa. Targeted coral species were collected to determine microbial species across variables such as (but not limited to): temperature, distance to river input, nearest population density, etc. In addition, corals were collected and a controlled experiment was conducted. To understand how differences in microbial community structure and function impacted coral survival, corals collected along the west coast of Okinawa were exposed to different stressors, for example temperature, pH, hypoxia, etc. Subsequent lab and data analyses are currently underway.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
I was able to conduct multiple fieldwork expeditions and accomplish a large experiment and subsequent sample processing. Currently, another tank experiment is underway. Processing of samples (DNA/RNA extraction) are underway, as well as data analyses.
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| 今後の研究の推進方策 |
This year I will conduct a short-term stress experiment (as a follow-up to a first round last year). Coral fragments from 3 coral species across 4 sites will be collected and transported back to the lab. A stress tank experiment will be conducted, and samples will be taken from all coral fragments pre- and post-stress. I will profile microbiomes (e.g., protists, archaea, bacteria, and viruses) of stressed and unstressed corals with amplicon and metagenome sequencing and apply RNA sequencing (RNA-Seq) to study the functional changes associated with stress. In addition, I will set up the reciprocal field transplant experiment and collect initial samples. I will process the samples mucus and tissue sampling, DNA extractions, RNA extractions, and sequencing will follow the same procedures as the short-term experiment.
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