| 研究実績の概要 |
In FY2021, we have investigated the expression of two key EMT regulators
Snai2 and Zeb2 in hemangioblast development. We have also mad expression constructs of these two genes (Snai2 and Zeb2) for functional analysis. In addition, we have analyzed the promoter regions of these two genes and identified the transcription start sites. With these data, we also cloned constructs for CRISPR-Cas9 mediated gene activation and inhibition for Snai2 and Zeb2.
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| 今後の研究の推進方策 |
In FY2022, we will analyze in detail the role of these two EMT regulators
(Snai2 and Zeb2) in regulating both hemangioblast fate (as assayed by several blood and endothelial markers, as we have used previously) and in regulating cell polarity markers of precursors (before blood and vessel fate
segregation) and during early lineage segregation (i.e., for the blood
lineage: when blood marker is being turned on, but before blood cells loose cell-cell contact with each other and with endothelial cells; for the endothelial lineage: when endothelial specific cell-cell junctional interactions are being established). By the end of FY2022, we expect to establish the timeline and hierarchy of EMT transcriptional regulation, lineage transcriptional regulation, and morphological features of blood and endothelial cells during the critical phase of their lineage specification and segregation.
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