| 研究実績の概要 |
In FY2022, we have performed experiments on effect of overexpression of Snai2 and Zeb2 in lineage contribution (blood, vessel, smooth muscle lineages). We have found that these EMT markers do not clearly affect lineage marker gene expression. However, they affect cell lineage segregation, likely after lineage specification. We have also investigated relationship between hemangioblast specifiers (NPAS4L and ETV2, two markers upstream of blood/vessel markers SCL/LMO2 and smooth muscle markers HAND1/2) and EMT regulators. As EMT occurs both before (during mesoderm formation) and after hemagioblast specification and at least SNAI2 is involved in both primitive streak EMT and lateral plate mesoderm EMT (subject of this project), we are in the process of sorting out epistatic relationship between SNAI2, NPAS4L, and SCL/LMO2/HAND2).
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| 今後の研究の推進方策 |
In FY2023, we will continue the project with the same direction outlined in our grant application. We will study relationship of EMT regulators (SNAI2 and ZEB2) and cell biological markers, including NCAM, a cell surface markers which we have found to correlated with hemangioblast differentiation, and VE-cadherin, a vascular specific adherens junction molecule marker endothelial type of epithelial organization. We aim to submit the results of this project for publication by the end of this fiscal year.
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