| 研究課題/領域番号 |
21K06292
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| 研究機関 | 東京都立大学 |
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研究代表者 |
クロニン アダム 東京都立大学, 理学研究科, 准教授 (30620489)
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| キーワード | social behaviour / immunity / social insects / evolutionary biology |
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| 研究実績の概要 |
We performed the first round of experiments on immune stimulus in social and solitary bees in Hokkaido. These bees were then shipped to Princeton University in the USA for RNAseq analysis of gene expression. However, due to poor sample preservation the resolution in sequencing was insufficient and only limited sequencing was undertaken as a result. Testing indicates that freezing in liquid nitrogen rather than using RNAlater resolves this issue, so the experiment will be repeated in 2022. We also collected Ceratina bees in Okinawa and hosted these in the laboratory successfully, where immune challenge and behavioural experiments were performed. A new bacterial assay method was used successfully to demonstrate differences in immune efficacy between social and solitary bees.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Despite problems with our RNAseq analysis, progress on the second front has been more successful than anticipated. Furthermore, we have developed a solution to the problem discovered in preservation which should ensure quality results are obtained in a second round of experimentation.
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| 今後の研究の推進方策 |
The following changes have been made to the research plan based on last year’s outcomes. Firstly, we will using shipping in liquid nitrogen to transfer all samples for RNAseq, as tests with RNAlater proved unsuccessful. This alternative method was tested in late 2021 and found to provide good results by our US collaborators. The associated costs with shipping can be accommodated with the current budget. Secondly, the new bacterial assay method was developed following discussions with Tsunaki Asano of the Cellular Genetics Laboratory at TMU and we will continue to work with this collaboration to perform immune testing. Finally, because of the success of the experimental approach in 2021, we plan to present these results earlier than anticipated, and will attend the international meeting of the Behavioural Ecology society in Sweden in 2022.
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| 次年度使用額が生じた理由 |
The following changes have been made to the research plan based on last year’s outcomes. Firstly, we will using shipping in liquid nitrogen to transfer all samples for RNAseq, as tests with RNAlater proved unsuccessful. This alternative method was tested in late 2021 and found to provide good results by our US collaborators. The associated costs with shipping can be accommodated with the current budget. Secondly, the new bacterial assay method was developed following discussions with Tsunaki Asano of the Cellular Genetics Laboratory at TMU and we will continue to work with this collaboration to perform immune testing. Finally, because of the success of the experimental approach in 2021, we plan to present these results earlier than anticipated, and will attend the international meeting of the Behavioural Ecology society in Sweden in 2022
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