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2021 年度 実施状況報告書

ATP-dependent liquid phase separation during aging and neurodegeneration

研究課題

研究課題/領域番号 21K06400
研究機関沖縄科学技術大学院大学

研究代表者

Guillaud Laurent  沖縄科学技術大学院大学, 分子神経科学ユニット, グループリーダー (90596222)

研究期間 (年度) 2021-04-01 – 2024-03-31
キーワードLiquid phase separation / Aging / Mitochondria / ATP / Neurodegeneration
研究実績の概要

We performed preliminary experiments on mouse primary neurons and human motor neurons from healthy individual and ALS patients currently available in lab. We measured mitochondria activity, ATP concentration and liquid phase separation of cGFP in mouse and human neurons and showed a significant reduction in ATP concentration in ALS neurons compared to healthy neuron, correlated to a decrease in LPS. We also tested the effect of NMN chronic treatment on mouse neurons and showed that 48h treatment induced increase of ATP in treated neurons compared to untreated neurons.

現在までの達成度 (区分)
現在までの達成度 (区分)

3: やや遅れている

理由

We had delay in selecting and acquiring human iPSC lines during the first year of the project but all the necessary lines are going to be purchase in the coming fiscal year.

今後の研究の推進方策

After acquisition and delivery of iPSC lines of different ages we will initiate their expansion and differentiation into motor neurons. After successful differentiation we will monitor and quantify mitochondria number, size and activity in motor neurons obtained from iPSCs of different ages. Concomitantly we will measure the axo-terminal and cell body cytosolic fluid phase by FRAP in motor neurons transduced with AAV-cGFP and expressing cytosolic GFP. And measure intracellular ATP concentration in neurons expressing PercevalHR (ATP fluorescent sensor) and by in vitro bioluminescence assay.

次年度使用額が生じた理由

We had delay to select and acquire the necessary human iPSC lines during the first year and the different lines will be purchased this coming fiscal year.

  • 研究成果

    (2件)

すべて 2021

すべて 雑誌論文 (1件) (うち国際共著 1件、 査読あり 1件、 オープンアクセス 1件) 学会発表 (1件) (うち招待講演 1件)

  • [雑誌論文] Development of 3D culture scaffolds for directional neuronal growth using 2-photon lithography2021

    • 著者名/発表者名
      Lokesh Agrawal, Menouer Saidani, Laurent Guillaud, Marco Terenzio
    • 雑誌名

      Materials Science & Engineering C

      巻: 131 ページ: -

    • DOI

      10.1016/j.msec.2021.112502

    • 査読あり / オープンアクセス / 国際共著
  • [学会発表] ATP hydrotropic regulation of neuronal proteins aggregation in synapse organization and neurodegeneration2021

    • 著者名/発表者名
      Laurent Guillaud
    • 学会等名
      Axonal Degeneration Regeneration International Workshop
    • 招待講演

URL: 

公開日: 2022-12-28  

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